Design/Advanced Electrostatic Sampler for Total Bioaerosols

设计/先进的总生物气溶胶静电采样器

• 批准号: 7270120
• 负责人: GEDIMINAS MAINELIS
• 金额: $ 17.41万
• 依托单位: RUTGERS, THE STATE UNIV OF N.J.
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-08-01 至 2009-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7270120
• 关键词: Design; Advanced; Electrostatic; Sampler; Total

DESCRIPTION (provided by applicant): To improve assessment and control to airborne microorganism exposure, and to protect the populations and resources at risk from airborne microbial agents advanced bioaerosol detection and sensing systems are needed. Commonly used bioaerosol samplers often measure viable bioaerosols which represent only a fraction of airborne microbial flora. In this research, responding to PA-04-030 "NIOSH Exploratory Developmental Grant Program (R21)", we propose to design, construct and evaluate a novel bioaerosol sampler which will improve our ability to determine exposures to total (viable and non-viable) airborne microorganisms. The proposed sampler will be compact, have low power consumption and, most importantly, will feature very high air sample concentration rate (1 million and higher). Such a high sample concentration rate is currently achieve by few other samplers and will significantly improve our ability to detect low airborne microorganism concentrations. In the proposed sampler, the airborne particles will be electrostatically deposited on a superhydrophobic surface ("Lotus leaf type) from which they will be removed and collected by tiny (10-50 /vl_) rolling liquid droplets, e.g. a phenomenon known as self-cleaning. The liquid droplets containing removed microorganisms will then be available for analysis by modern microbiological techniques, such as Real-Time Polymerase Chain Reaction (RT-PCR). The new sampler's overall performance (sample collection, extraction, and its subsequent analysis by RT-PCR) when collecting test bacteria and fungi will be evaluated in laboratory and field settings and against other samplers. The development of the electrostatic collector with superhydrophobic collection surface will improve our ability to determine exposure to airborne microorganisms in residential, occupational and environmental settings, thus improving our ability to protect populations are risk. The use of small collection liquid quantities will make the sampler compatible with various "laboratories on a chip" and may lead to near real-time determination of airborne microbial contaminants. In addition, use of electrostatic technique as a particle removal mechanism will enable collection and detection of airborne viruses, such as SARS or monkey pox. The research findings and practical recommendations developed in this research will be disseminated to stakeholders through internet, national and international conferences and workshops.

描述（由申请人提供）：为了改善空气中微生物的评估和控制，并保护有风险的人群和资源免受空气中的微生物剂，需要先进的生物素检测和传感系统。常用的生物溶质溶液采样器通常测量仅代表空气中微生物菌群的一小部分的可行生物溶质。在这项研究中，我们建议对PA-04-04-030“ NIOSH探索性发展赠款计划（R21）”，我们建议设计，构建和评估一种新型的Bioaerosol Sampler，这将提高我们确定总（可行和不可行的）飞机微生物的接触能力。所提出的采样器将紧凑，功耗低，最重要的是，空气样品浓度率很高（100万及更高）。目前，其他很少的采样器实现了如此高的样本浓度率，将显着提高我们检测到低空微生物浓度的能力。在拟议的采样器中，空气中的颗粒将被静电沉积在超疏水表面（“莲花叶型）上，将它们通过微小（10-50 /vl_）滚动液滴的微小（10-50 /vl_）滚动，从而收集并收集它们，例如，由液态液滴被称为自动液滴。当收集测试细菌和真菌评估新样本的整体性能，随后的RT-PCR（RT-PCR）。因此，提高我们保护人口的能力是风险。另外，将静电技术用作颗粒去除机制将使空气传播病毒（例如SARS或Monkey Pox）的收集和检测。这项研究中提出的研究发现和实际建议将通过互联网，国家和国际会议和研讨会传播给利益相关者。