A novel passive sampler to assess personal exposures to airborne biological agents

一种新型被动采样器，用于评估个人接触空气中生物制剂的情况

• 批准号: 10647398
• 负责人: GEDIMINAS MAINELIS
• 金额: $ 22.18万
• 依托单位: RUTGERS, THE STATE UNIV OF N.J.
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-09-01 至 2025-08-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10647398
• 关键词: novel; passive; sampler; assess; personal

A novel passive sampler to assess personal exposures to airborne biological agents This grant application responds to PAR-18-798: NIOSH Exploratory/Developmental Grant Program (R21). We propose developing and testing a qualitatively new personal sampling device to monitor personal exposures to bioaerosols without needing a pump or power source while being lightweight, unobtrusive, and cheap. These advances are enabled by using novel materials and innovative sampler design. In addition, this novel personal sampling concept is strongly supported by preliminary data. We hypothesize that Personal Rutgers Electrostatic Passive Sampler (P-REPS) will have a sampling performance comparable to existing active personal samplers while offering light weight, small footprint, low cost, absence of pumps or power supplies, and sampling timeframes as long as days and even weeks. The sampler will help us better understand personal exposures to airborne biological agents and the transmission of those agents in occupational environments. P-REPS will use an electrostatic field created by specially configured poly(vinylidene fluoride) (PVDF) film, a permanently polarized ferroelectric polymer, to capture bioaerosol particles. The PVDF film will be wound in a spiral configuration so that the film surfaces with opposing polarization face one another and create a strong electrostatic field. The electrostatic field between the film layers and its fringing effect will attract bioaerosol and deposit them onto the film for post-sampling elution and analysis. The film will be secured in a 3D-printed frame, protective housing, and a slotted umbrella-shaped cap. Once the sampler is manufactured, it will be tested in chamber studies against an active personal sampler using several airborne microorganisms. The organisms captured by both samplers will be examined using microscopy, culturing, and ATP analysis and their quantity will be compared. The data will be used to calculate the Equivalent Sampling Rate (ESR) of P-REPS – a flow rate at which a filter sampler would capture the same number of microorganisms. Next, P-REPS will be tested against a reference sampler in several field environments with different microorganism concentrations. Here, both samplers will be placed on rotating manikins and also worn by study personnel. Our goal is to achieve ESR similar to or better than the flow rate of an active personal filter-based sampler, such as the IOM sampler. The proposal responds to NIOSH Strategic Plan’s “Immune, Infectious, and Dermal Disease Prevention” cross-sector and sectors of National Agriculture, Forestry, and Fishing; Healthcare; Services; Transportation, Warehousing, and Utilities. This proposal responds to the NIOSH r2P initiative by developing an innovative personal sampler to determine personal exposures to bioaerosols, especially over long time frames, thus leading to worker health protection. The output of the study will be a prototype P-REPS for determining personal exposures to respiratory agents in occupational environments. We expect that intermediate outcomes will include a patent, citations in the literature, and, finally, the adaptation of the sampler in work environments. The end outcome is expected to be a better understanding of personal exposure to bioaerosols in workplaces.

一种新型被动采样器，用于评估个人接触空气中生物制剂的情况 该拨款申请响应 PAR-18-798：NIOSH 探索/发展拨款计划 (R21)。 开发和测试一种新的定性个人采样建议装置，以监测个人接触 生物气溶胶不需要泵或电源，同时重量轻、不引人注目且便宜。 此外，这种新颖的个人化技术还通过使用新颖的材料和创新的采样器设计来实现。 初步数据有力地支持了采样概念。我们捕获了罗格斯个人静电。 被动采样器 (P-REPS) 的采样性能可与现有主动个人采样器相媲美 同时重量轻、占地面积小、成本低、无需泵或电源以及采样 采样器的时间范围长达几天甚至几周，将帮助我们更好地了解个人暴露情况。 空气中的生物制剂以及这些制剂在职业环境中的传播。 P-REPS 将使用由特殊配置的聚偏二氟乙烯 (PVDF) 薄膜创建的静电场， 永久极化的铁电聚合物，用于捕获生物气溶胶颗粒，将缠绕在一个容器中。 螺旋结构，使具有相反偏振的薄膜表面彼此相对并形成强大的 静电场 膜层之间的静电场及其边缘效应会吸引生物气溶胶和 将它们沉积到胶片上进行采样后洗脱和分析。胶片将固定在 3D 打印的框架中， 采样器制造完成后，将在内部进行测试。 使用几种空气传播的微生物对主动个人采样器进行室研究。 两个采样器捕获的样本将通过显微镜、培养和 ATP 分析及其数量进行检查 数据将用于计算 P-REPS 的等效采样率 (ESR) – 流。 过滤采样器捕获相同数量微生物的速率 接下来，将测试 P-REPS。 与不同微生物浓度的几种现场环境中的参考采样器相比， 两个采样器都将放置在旋转人体模型上，并由研究人员佩戴。我们的目标是实现 ESR。 类似于或优于基于主动个人过滤器的采样器（例如 IOM 采样器）的流速。 该提案响应了 NIOSH 战略计划的“免疫、传染病和皮肤病预防” 国家农业、林业和渔业服务的跨部门和部门； 仓储和公用事业 该提案通过开发创新来响应 NIOSH r2P 倡议。 个人采样器可确定个人对生物气溶胶的暴露情况，尤其是在较长时间内，从而导致 该研究的输出将是用于确定个人健康的 P-REPS 原型。 我们预计，中间结果将包括在职业环境中接触呼吸性病原体。 专利、文献引用，最后是采样器在工作环境中的适应性。 预计结果将是更好地了解个人在工作场所接触生物气溶胶的情况。