A novel passive sampler to assess personal exposures to airborne biological agents

一种新型被动采样器，用于评估个人接触空气中生物制剂的情况

• 批准号: 10647398
• 负责人: GEDIMINAS MAINELIS
• 金额: $ 22.18万
• 依托单位: RUTGERS, THE STATE UNIV OF N.J.
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-09-01 至 2025-08-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10647398
• 关键词: novel; passive; sampler; assess; personal; novel; passive; sampler; assess; personal

A novel passive sampler to assess personal exposures to airborne biological agents This grant application responds to PAR-18-798: NIOSH Exploratory/Developmental Grant Program (R21). We propose developing and testing a qualitatively new personal sampling device to monitor personal exposures to bioaerosols without needing a pump or power source while being lightweight, unobtrusive, and cheap. These advances are enabled by using novel materials and innovative sampler design. In addition, this novel personal sampling concept is strongly supported by preliminary data. We hypothesize that Personal Rutgers Electrostatic Passive Sampler (P-REPS) will have a sampling performance comparable to existing active personal samplers while offering light weight, small footprint, low cost, absence of pumps or power supplies, and sampling timeframes as long as days and even weeks. The sampler will help us better understand personal exposures to airborne biological agents and the transmission of those agents in occupational environments. P-REPS will use an electrostatic field created by specially configured poly(vinylidene fluoride) (PVDF) film, a permanently polarized ferroelectric polymer, to capture bioaerosol particles. The PVDF film will be wound in a spiral configuration so that the film surfaces with opposing polarization face one another and create a strong electrostatic field. The electrostatic field between the film layers and its fringing effect will attract bioaerosol and deposit them onto the film for post-sampling elution and analysis. The film will be secured in a 3D-printed frame, protective housing, and a slotted umbrella-shaped cap. Once the sampler is manufactured, it will be tested in chamber studies against an active personal sampler using several airborne microorganisms. The organisms captured by both samplers will be examined using microscopy, culturing, and ATP analysis and their quantity will be compared. The data will be used to calculate the Equivalent Sampling Rate (ESR) of P-REPS – a flow rate at which a filter sampler would capture the same number of microorganisms. Next, P-REPS will be tested against a reference sampler in several field environments with different microorganism concentrations. Here, both samplers will be placed on rotating manikins and also worn by study personnel. Our goal is to achieve ESR similar to or better than the flow rate of an active personal filter-based sampler, such as the IOM sampler. The proposal responds to NIOSH Strategic Plan’s “Immune, Infectious, and Dermal Disease Prevention” cross-sector and sectors of National Agriculture, Forestry, and Fishing; Healthcare; Services; Transportation, Warehousing, and Utilities. This proposal responds to the NIOSH r2P initiative by developing an innovative personal sampler to determine personal exposures to bioaerosols, especially over long time frames, thus leading to worker health protection. The output of the study will be a prototype P-REPS for determining personal exposures to respiratory agents in occupational environments. We expect that intermediate outcomes will include a patent, citations in the literature, and, finally, the adaptation of the sampler in work environments. The end outcome is expected to be a better understanding of personal exposure to bioaerosols in workplaces.

一个新颖的被动抽样器，以评估个人接触机载生物剂 该赠款申请对PAR-18-798的响应：NIOSH探索/发展赠款计划（R21）。我们 提案开发和测试一种定性新的个人抽样设备，以监视个人暴露 在轻巧，不引人注目且便宜的同时，无需泵或电源。这些 使用新颖的材料和创新的采样器设计可以实现进步。此外，这本小说个人 初步数据强烈支持采样概念。我们假设个人罗格静电 被动采样器（P-REPS）将具有与现有主动个人样本相当的抽样性能 在提供轻巧，小足迹，低成本，缺少泵或电源的同时以及采样 时间范围只有几天甚至几周。采样器将帮助我们更好地了解个人接触 机载生物制剂和这些特工在占领环境中的传播。 P-REP将使用特殊配置的聚（乙烯基氟化物）（PVDF）膜创建的静电场 永久极化的铁电聚合物，以捕获生物溶质颗粒。 PVDF电影将在 螺旋构型以使胶片与相反的两极分化相互面对并产生强大 静电场。薄膜层及其条纹效果之间的静电场将吸引生物美感和 将它们存放到膜上进行抽样后的洗脱和分析。这部电影将在3D打印的框架中固定 保护性外壳和一个开槽的伞状盖。一旦制造采样器，将在 使用几种机载微生物对主动的个人采样器进行的室内研究。生物 通过显微镜，文化和ATP分析将检查两个样品捕获的及其数量 将被比较。数据将用于计算P-REP的等效采样率（ESR） - 流量 过滤器采样器将捕获相同数量的微生物的速率。接下来，P-REP将进行测试 针对具有不同微生物浓度的几个田间环境中的参考采样器。这里， 这两个样本都将用于旋转的手持蛋白，并由研究人员佩戴。我们的目标是实现ESR 类似于或更好的是主动滤波器采样器（例如IOM采样器）的流速。 该提案回应了NIOSH战略计划的“免疫，传染性和皮肤病预防” 国家农业，林业和捕鱼的跨部门和部门；卫生保健;服务；运输， 仓库和公用事业。该提案通过开发创新性来回应NIOSH R2P计划 个人抽样器以确定个人暴露于生物美感，尤其是长期框架，从而领先 进行工人健康保护。研究的输出将是用于确定个人的原型P-REP 暴露于占用环境中的呼吸剂。我们预计中级结果将包括 文献中的专利，引用，最后是在工作环境中采样器的适应。结尾 预计结果将更好地理解工作场所中对生物美感的个人接触。