Imaging Core

成像核心

• 批准号: 7081711
• 负责人: ELLEN A ROBEY
• 金额: $ 41.7万
• 依托单位: UNIVERSITY OF CALIFORNIA BERKELEY
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-07-15 至 2011-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7081711
• 关键词: bioimaging /biomedical imaging; biomedical facility; cellular immunity; computer data analysis; disease /disorder model; experimental designs; fluorescence microscopy; host organism interaction; image processing; imaging /visualization /scanning; intravital microscopy; laboratory mouse; microorganism immunology; molecular /cellular imaging; parasite infection mechanism; virus infection mechanism

Two-photon imaging can provide dynamic information about the behavior of pathogens and immune cells in tissues during infection. Such information can provide unique insight into the course of events during infection and the impact of therapeutic treatments. However, this technique is outside the reach of many investigators due to the cost of purchasing and maintaining equipment, and the challenges in preparing samples and analyzing data. In this proposal we plan to make this powerful tool available to the investigators of the PO1 by: (1) working with individual labs on pilot studies to determine the optimal experimental set-ups to meet individual imaging goal, (2) expanding existing imaging equipment to provide more access, and (3) assisting individual labs to make use of quantitation software and perform mathematical analysis of visual data.

两光子成像可以提供有关病原体和免疫细胞行为的动态信息 感染过程中的组织。此类信息可以为事件过程提供独特的见解 感染和治疗治疗的影响。但是，这项技术超出了许多人的范围 调查人员由于购买和维护设备的成本以及准备的挑战 样本和分析数据。在此提案中，我们计划使该功能强大的工具可用于 PO1的研究者：（1）与单个实验室合作进行试验研究以确定最佳 实验设置以满足单个成像目标，（2）扩展现有成像设备以提供 更多访问权限，（3）协助单个实验室使用定量软件并执行 视觉数据的数学分析。