Imaging Core

成像核心

• 批准号: 7081711
• 负责人: ELLEN A ROBEY
• 金额: $ 41.7万
• 依托单位: UNIVERSITY OF CALIFORNIA BERKELEY
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-07-15 至 2011-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7081711
• 关键词: bioimaging /biomedical imaging; biomedical facility; cellular immunity; computer data analysis; disease /disorder model; experimental designs; fluorescence microscopy; host organism interaction; image processing; imaging /visualization /scanning; intravital microscopy; laboratory mouse; microorganism immunology; molecular /cellular imaging; parasite infection mechanism; virus infection mechanism

Two-photon imaging can provide dynamic information about the behavior of pathogens and immune cells in tissues during infection. Such information can provide unique insight into the course of events during infection and the impact of therapeutic treatments. However, this technique is outside the reach of many investigators due to the cost of purchasing and maintaining equipment, and the challenges in preparing samples and analyzing data. In this proposal we plan to make this powerful tool available to the investigators of the PO1 by: (1) working with individual labs on pilot studies to determine the optimal experimental set-ups to meet individual imaging goal, (2) expanding existing imaging equipment to provide more access, and (3) assisting individual labs to make use of quantitation software and perform mathematical analysis of visual data.

双光子成像可以提供有关病原体和免疫细胞行为的动态信息 感染期间的组织。这些信息可以提供对事件进程的独特见解 感染和治疗的影响。然而，这项技术超出了许多人的能力范围 由于购买和维护设备的成本以及准备工作的挑战，调查人员 样品和分析数据。在本提案中，我们计划将这个强大的工具提供给 PO1 的研究人员通过以下方式进行：(1) 与各个实验室合作进行试点研究以确定最佳方案 满足个人成像目标的实验装置，（2）扩展现有成像设备以提供 更多的访问权限，以及 (3) 协助各个实验室利用定量软件并执行 视觉数据的数学分析。