Core B - Animal Exposure and Assessment

核心 B - 动物暴露和评估

• 批准号: 7089298
• 负责人: CHARLES George PLOPPER
• 金额: $ 53.1万
• 依托单位: UNIVERSITY OF CALIFORNIA AT DAVIS
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-04-01 至 2011-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7089298
• 关键词: Macaca mulatta; air pollution; animal care; animal colony; biomedical facility; environmental exposure; environmental health; environmental toxicology; pollution related respiratory disorder; statistical service /center

The overall goal of the Animal Exposure and Assessment Core is to provide support to investigators studying the impact of oxidant air pollutants on allergic airways disease in infant rhesus monkeys. The Core is housed in the Exposure Facility at the California National Primate Research Center (CNPRC). Our Program Project team has recently established a non-human primate model of allergic airways disease that closely matches human infant allergic airways disease. Our model is generated in rhesus monkeys using a known human allergen, the house dust mite (HDM; Dermatophagoides famiae). We have found that ozone (O3) co-exposure markedly enhances the disease. The Exposure Facility at the CNPRC has well-established facilities for assessing all aspects of airways reactivity, immune response, and exposure to allergens and oxidant gases with validated exposure conditions, and it has a complete pulmonary function testing unit. Specifically, this Core will: 1. Provide accurate and carefully controlled exposures of animals or in vitro preparations to selected types and concentrations of air pollutants. 2. Develop methods of inhalation exposure and atmosphere characterization. 3. Coordinate the purchase, health services, and care of experimental animals. 4. Provide logistical and technical support to ensure the correct implementation of experimental regimens. 5. Prepare HDM allergen for use in sensitization and challenge of infant rhesus monkeys. 6. Sensitize infant rhesus monkeys to HDM allergen using a published protocol developed by our group. 7. Verify sensitization to HDM allergen using skin prick tests. 8. Provide researchers with infant rhesus monkeys with carefully defined allergic airways disease using carefully controlled and validated exposure conditions for oxidant gases and HDM allergen. 9. Provide carefully documented status of airway responsiveness and lung mechanics and volumes in infant rhesus monkeys. 10. Collate all exposure, sensitization, and pulmonary function data and provide this to the experimental units. 11. Coordinate the acquisition of tissues at necropsy and maintain the collection archive and sample custody and storage log.

动物暴露和评估核心的总体目标是为研究人员提供支持 氧化剂空气污染物对婴儿恒河猴过敏气道疾病的影响。核心安置在 加利福尼亚国家灵长类动物研究中心（CNPRC）的接触设施。我们的计划项目团队 最近已经建立了一种非人类灵长类动物模型的过敏气道疾病，与人类密切相匹配 婴儿过敏性气道疾病。我们的模型是使用已知的人过敏原的恒河猴生成的 房屋尘螨（HDM； Dermatophagoides famiae）。我们发现臭氧（O3）共同暴露明显 增强疾病。 CNPRC的曝光设施拥有完善的设施来评估所有 气道反应性，免疫反应以及对过敏原和氧化气的暴露的各个方面 暴露条件，并具有完整的肺功能测试单元。具体而言，此核心将： 1。为选定提供准确，仔细控制的动物或体外制剂 空气污染物的类型和浓度。 2。开发吸入暴露和大气表征的方法。 3。协调购买，卫生服务和实验动物的护理。 4.提供后勤和技术支持，以确保正确实施实验方案。 5。准备HDM过敏原以用于婴儿恒河猴的敏化和挑战。 6。使用我们小组开发的公开方案使婴儿恒河猴对HDM过敏原敏感。 7。使用皮肤刺测试验证对HDM过敏原的敏化。 8。为研究人员提供婴儿恒河猴，并使用精心定义的过敏性气道疾病 精心控制和验证的氧化气和HDM过敏原的暴露条件。 9。提供仔细记录的气道响应状态，肺部力学和量 婴儿恒河猴。 10。整理所有暴露，敏化和肺功能数据，并将其提供给实验 单位。 11。协调在尸检处的组织的获取并维护收集档案和样品 保管和存储日志。