Targeted, multimodal detection of carbon monoxide and other markers of disease in cells using fluorogenic probes

使用荧光探针对细胞中一氧化碳和其他疾病标志物进行靶向多模式检测

• 批准号: 2897624
• 金额: --
• 依托单位: Imperial College London
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2023
• 资助国家: 英国
• 起止时间: 2023 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2897624
• 关键词: Targeted; multimodal; detection; carbon; monoxide

Despite its toxic reputation, carbon monoxide (CO) is also an important biological messenger molecule that regulates many vital cell processes, including the response to disease. Increased enzymatic generation of carbon monoxide plays a critical role in the resolution of inflammatory processes and alleviation of cardiovascular disorders. At the same time, altered viscosity levels have been associated with inflammation, including in cardiovascular disease. This project will develop a range of ruthenium(II) and osmium(II) molecular probes for carbon monoxide and has used them to monitor inflammation in an animal model. Since the diffusion-controlled movement of carbon monoxide is also affected by changes in the viscosity of the cellular environment, the two aspects are intimately connected. Therefore, the simultaneous measurement of both carbon monoxide and other markers of disease, such as intracellular viscosity, provides unprecedented information on the functioning of the cell and the state of inflammation and disease.

尽管具有毒性的声誉，但一氧化碳（CO）还是重要的生物允许分子，可调节许多重要的细胞过程，包括对疾病的反应。增加的一氧化碳酶产生在炎症过程和减轻心血管疾病的方法中起着至关重要的作用。同时，粘度水平改变与炎症有关，包括心血管疾病。该项目将开发一系列的一氧化碳（II）和osmium（II）分子探针，并使用它们用于监测动物模型中的炎症。由于一氧化碳的扩散控制的运动也受细胞环境粘度变化的影响，因此这两个方面紧密相连。因此，同时测量了一氧化碳和其他疾病标志物（例如细胞内粘度），提供了有关细胞功能以及炎症和疾病状态的前所未有的信息。