Biophysical studies of a Sulfolobus icosahedral virus

硫化叶菌二十面体病毒的生物物理学研究

• 批准号: 6937283
• 负责人: Reza Khayat
• 金额: $ 4.4万
• 依托单位: SCRIPPS RESEARCH INSTITUTE, THE
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-04-01 至 2008-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6937283
• 关键词: Adenoviridae; Archaea; algae; bacterial virus; biochemical evolution; biophysics; capsid; cryoelectron microscopy; host organism interaction; postdoctoral investigator; protein structure function; radiography; virus infection mechanism; virus protein

DESCRIPTION (provided by applicant): The Sulfolobus Turreted Icosahedral virus (STIV) is a lytic hyperthermophile virus that infects members of the Sulfolobus family within the kingdom Crenarchaeota of the domain Archaea. The cryo-electron microscope (Cryo-EM) image reconstruction of the virus shows a striking architectural resemblance to the mammalian adenovirus, the bacteriophage PRD1, and algal virus PBCV-1. Structural studies of the major capsid proteins of adenovirus, PRD1, and PBCV-1 have shown a strong possiblity of evolutionary relationship between these viruses. This proposal sets forth structural and biophysical studies aimed in characterizing STIV and identifying whether this virus can be evolutionary related to the above mentioned viruses. The identification of a virus phylogeny encompassing the three divisions of life is exciting and unprecedented. Characterization of STIV, and Archaea infecting viruses in general, will provide substantial insight into the biochemistry of Archaea, virus host relationship and evolution, and biochemical adaptations required for survival under extreme environments. Detailed characterization of Archaea viruses may also expand our knowledge of viral evolution.

描述（由申请人提供）： 硫化叶菌转塔二十面体病毒 (STIV) 是一种裂解性超嗜热病毒，可感染古菌域泉弧菌界内的硫化叶菌家族成员。该病毒的冷冻电子显微镜 (Cryo-EM) 图像重建显示其结构与哺乳动物腺病毒、噬菌体 PRD1 和藻类病毒 PBCV-1 具有惊人的相似性。对腺病毒、PRD1 和 PBCV-1 的主要衣壳蛋白的结构研究表明，这些病毒之间极有可能存在进化关系。该提案提出了结构和生物物理研究，旨在表征 STIV 并确定该病毒是否与上述病毒具有进化相关性。涵盖生命三个部分的病毒系统发育的鉴定是令人兴奋且前所未有的。 STIV 和古细菌感染病毒的表征将为古细菌的生物化学、病毒宿主关系和进化以及极端环境下生存所需的生化适应提供深入的了解。古细菌病毒的详细表征也可能会扩展我们对病毒进化的了解。