Dissecting heat stress responses in barley

剖析大麦的热应激反应

• 批准号: 2886133
• 金额: --
• 依托单位: University of Nottingham
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2023
• 资助国家: 英国
• 起止时间: 2023 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2886133
• 关键词: Dissecting; heat; stress; responses; barley

Background: Impact of changing climatic conditions on global crop production. We need to increase the population to feed the world but while reducing the reliance on fertiliser application. One solution is to develop varieties that have improved soil resource capture and thus improved yield under suboptimal conditions. Crop yields are globally affected by abiotic stresses such as drought, salinity and waterlogging. Ubiquitin-mediated proteolysis via the Plant Cysteine Oxidase (PCO) branch of the PRT6 N-Degron pathway controls the plant response by regulating the turnover of proteins involved in sensing and/or conferring tolerance to abiotic stresses. Root cortical senescence (RCS). Importance of cortical senescence towards this especially improving yield while reducing metabolic burden of maintaining cortical tissues under abiotic stresses. RCS is a very important breeding target for edaphic stress tolerance. The fundamental understanding about genes and mechanisms controlling this process is missing. Interestingly, we recently observed that mutants in PRT6 N-degron pathway have increased RCS. This project focuses on newly discovered and uncharacterized N-Degron pathway substrates to shed light on their specific downstream stress regulation functions, offering new opportunities for stress resilience research. We have previously identified two such substrates, BERF1 and RAF; this project will study their specific roles as key regulators of environmental stress. A recent paper showed RAF and BERF1 were upregulated in a transcriptional analysis, which indicated their important role of these gene in the development of root cortical senescence (RCS). Thus, in this DTP project we aim to dissect this to improve our fundamental understanding about genes controlling this process and identify novel genetic components/solutions that could help us develop varieties tolerant to abiotic stresses.Work plan: Year 1: Map the development of root cortical senescence during drought in the mutant and WT. Decide zones and timepoints for sampling roots and perform RNAseq of mutant vs WT under control and drought stress and sample different zones, including RCS formation to understand genes expressed. Prioritise some candidates' genes based on previous work and its relevance in drought, etc.Year 2-3: Develop CRISPR, RNAi, reporter lines to validate downstream targets of PRT6 N-degron pathway functioning in regulating root cortical senescence. Year 3-4. Perform drought experiment in soil columns in Hounsfield facility. Harvest roots for Laser ablation tomography and see whether RCS formation is impaired in the mutant and its impact on the cost benefit of forming RCS in the mutant vs WT. Also study shoot physiological parameters using PhenoSpex trait finder in the Hounsfield facility. Year 4: Deploy novel genetic solutions to breeding companies. Project output and impact: This project focuses on newly discovered and uncharacterized N-Degron pathway substrates to shed light on their specific downstream stress regulation functions, offering new opportunities for stress resilience research. This project therefore combines fundamental research into understanding the drivers for abiotic stress resilience, with application to generate resilient barley for deployment.

背景：气候条件变化对全球作物生产的影响。我们需要增加人口来养活世界，同时减少对化肥施用的依赖。一种解决方案是开发能够改善土壤资源捕获的品种，从而在次优条件下提高产量。全球农作物产量受到干旱、盐碱和涝害等非生物胁迫的影响。通过 PRT6 N-Degron 途径的植物半胱氨酸氧化酶 (PCO) 分支进行的泛素介导的蛋白水解作用，通过调节参与感知和/或赋予非生物胁迫耐受性的蛋白质的周转来控制植物反应。根皮质衰老（RCS）。皮质衰老对此的重要性，特别是提高产量，同时减少在非生物胁迫下维持皮质组织的代谢负担。 RCS是土壤胁迫耐受性的一个非常重要的育种目标。人们对控制这一过程的基因和机制缺乏基本的了解。有趣的是，我们最近观察到 PRT6 N-degron 通路中的突变体 RCS 增加。该项目重点关注新发现和未表征的 N-Degron 通路底物，以阐明其特定的下游应激调节功能，为应激恢复研究提供新的机会。我们之前已经鉴定了两种这样的底物：BERF1 和 RAF；该项目将研究它们作为环境压力关键调节者的具体作用。最近的一篇论文表明，RAF 和 BERF1 在转录分析中上调，这表明这些基因在根皮层衰老 (RCS) 发展中的重要作用。因此，在这个 DTP 项目中，我们的目标是对此进行剖析，以提高我们对控制这一过程的基因的基本理解，并确定新的遗传成分/解决方案，帮助我们开发耐受非生物胁迫的品种。工作计划：第一年：绘制根部发育图突变体和野生型干旱期间皮质衰老。确定根部采样的区域和时间点，并在控制和干旱胁迫下对突变体与野生型进行 RNAseq，并对不同区域进行采样，包括 RCS 形成以了解表达的基因。根据之前的工作及其与干旱等的相关性，优先考虑一些候选基因。第2-3年：开发CRISPR、RNAi、报告系，以验证PRT6 N-degron途径下游靶点在调节根皮层衰老中的功能。第 3-4 年。在亨斯菲尔德设施的土柱中进行干旱实验。收获根进行激光消融断层扫描，观察突变体中 RCS 的形成是否受到损害，及其对突变体与 WT 中形成 RCS 的成本效益的影响。还使用 Hounsfield 设施中的 PhenoSpex 性状查找器研究芽生理参数。第四年：为育种公司部署新颖的遗传解决方案。项目成果和影响：该项目重点关注新发现和未表征的N-Degron通路底物，以阐明其特定的下游应激调节功能，为应激恢复研究提供新的机会。因此，该项目将基础研究结合起来，以了解非生物胁迫恢复力的驱动因素，并应用产生用于部署的恢复力大麦。