Circuit Mechanisms of Cortical Synchronizations

皮质同步的电路机制

• 批准号: 7047716
• 负责人: Brendon O Watson
• 金额: $ 3.49万
• 依托单位: COLUMBIA UNIVERSITY HEALTH SCIENCES
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-07-01 至 2010-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7047716
• 关键词: action potentials; brain electrical activity; brain mapping; cell population study; cerebral cortex; electrophysiology; fluorescence microscopy; fluorescent dye /probe; genetically modified animals; glutamates; laboratory mouse; lasers; neural information processing; neural transmission; neurons; predoctoral investigator; thalamus; tissue /cell culture; voltage /patch clamp

DESCRIPTION (provided by applicant): The proposed studies are aimed at elucidating the cellular mechanisms within cortex that lead to the generation of UP states. UP states are a distinct type of synchronous activation of neuronal ensembles in mammalian brain in which groups of neurons are synchronously depolarized for hundreds of milliseconds and fire action potentials during that time. They have been associated with neural function such a sensory input and motor output. As importantly, the ensemble of neurons participating in an UP state appears to be stable, in that the same local group of cells fires in the same sequence on repeated occasions. The stereotyped spatiotemporal dynamics of these neural ensembles suggest that they may arise from underlying neuronal connectivity patterns that are critical for their organization and function. However, much remains to be learned about how these UP states are triggered or maintained. This application tests the hypothesis that neurons that are members of UP state ensembles are themselves critical for triggering and maintaining their own synchronous depolarization. We propose a series of stimulation and ablation studies to test this hypothesis. Given the possibility that these neural ensembles likely represent modular elements critical in cortical function, their disruption may lead to disorganization of neural activity as is observed in a number of neurological disorders including epilepsy.

描述（由申请人提供）： 拟议的研究旨在阐明皮层内导致 UP 状态产生的细胞机制。 UP 状态是哺乳动物大脑中神经元群同步激活的一种独特类型，其中神经元组同步去极化数百毫秒，并在此期间激发动作电位。它们与感觉输入和运动输出等神经功能有关。同样重要的是，参与 UP 状态的神经元集合似乎是稳定的，因为同一局部细胞组在重复的情况下以相同的顺序放电。这些神经集合的刻板时空动力学表明它们可能源于对其组织和功能至关重要的潜在神经元连接模式。然而，关于这些 UP 状态是如何触发或维持的，还有很多东西有待了解。该应用测试了这样的假设：作为 UP 状态集合成员的神经元本身对于触发和维持其自身的同步去极化至关重要。我们提出了一系列刺激和消融研究来检验这一假设。考虑到这些神经系统可能代表了对皮质功能至关重要的模块元件，它们的破坏可能会导致神经活动的紊乱，正如在包括癫痫在内的许多神经系统疾病中观察到的那样。