Linear Ion Trap for Proteomics

用于蛋白质组学的线性离子阱

• 批准号: 7047361
• 负责人: PAUL M STEMMER
• 金额: $ 33.7万
• 依托单位: WAYNE STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-04-01 至 2007-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7047361
• 关键词: ions; proteins; proteomics; university

DESCRIPTION (provided by applicant): The goal of this proposal is to bring a LTQ-11000 Linear Ion Trap Mass Spectrometer with 2D nan-LC to Wayne State University. This instrument, designed to perform peptide sequence analysis and to identify and localize post-translational modifications in proteins, will be a major asset to research programs of NIH-funded investigators at WSU whose research is focused primarily on the regulation of cellular signal transduction pathways in healthy and diseased states. It is well established that control of these pathways is dependent on protein interactions and regulated changes in enzyme activities, many of which are modulated by post- translation modifications. Primary Users of the proposed LTQ Linear Ion Trap Mass Spectrometer work on signaling pathways that are modulated by phosphorylation or oxidation. Their research requires identification of proteins and analysis of the post-translational modifications of those proteins in response to specific stimuli and/or in specific disease states. The only unequivocal way to address these questions is through mass spectrometry. There is no MS/MS instrument at Wayne State or in the metropolitan Detroit area that can be used for proteomic analysis. The Proteomics Facility Core within the EHS Center at WSU has been established as the first source for these proteomic services at Wayne State. The University is strongly committed to this proposal as evidenced by the following support: 1) A funded long term plan for maintenance and operation of the LTQ Linear Ion Trap MS. 2) Dedicated laboratory space for a Proteomic Core Facility that will house the instrument. 3) Funding for salary and training of a technician to operate and schedule usage of the instrument. The LTQ-11000 Linear Ion Trap Mass Spectrometer is a state-of-the-art system with unparalleled speed and the ability to detect and localize post-translational modifications in proteins. At WSU, this instrument will allow more productive use of instrumentation already in place for proteomic fractionation and will provide efficient access to modern proteomic analysis for NIH-funded investigators who are now limited by the lack of local proteomic support.

描述（由申请人提供）：该提案的目的是将带有2D NAN-LC的LTQ-11000线性离子陷阱质谱仪带到韦恩州立大学。该仪器旨在进行肽序列分析，并识别和定位蛋白质中的翻译后修饰，将成为WSU NIH资助的研究人员研究计划的主要资产，其研究主要集中在健康和患病状态中细胞信号转导途径的调节。众所周知，对这些途径的控制取决于蛋白质相互作用和酶活性的调节变化，其中许多是通过翻译后修改调节的。所提出的LTQ线性离子陷阱质谱仪的主要用户在通过磷酸化或氧化调节的信号通路上工作。他们的研究需要鉴定蛋白质，并分析这些蛋白质的翻译后修饰，以响应特定的刺激和/或特定疾病状态。解决这些问题的唯一明确方法是通过质谱法。韦恩州或大都会底特律地区没有MS/MS仪器可用于蛋白质组学分析。 WSU EHS中心内的蛋白质组学设施核心已成为韦恩州这些蛋白质组学服务的第一个来源。该大学强烈致力于这项提案，这是由以下支持证明的：1）资助的LTQ线性离子陷阱MS维护和操作的长期计划。 2）专门的实验室空间，用于容纳该仪器的蛋白质组学核心设施。 3）用于薪水和培训技术人员的资金，以操作和安排该工具的使用。 LTQ-11000线性离子陷阱质谱仪是一个最先进的系统，具有无与伦比的速度，并且能够检测和定位蛋白质中翻译后修饰。在WSU，该仪器将允许对已经进行蛋白质组学分馏的仪器进行更高的生产力，并为NIH资助的研究人员提供有效的现代蛋白质组学分析，这些研究人员现在受到缺乏局部蛋白质组学支持的限制。