CORE-- Protein Interactions and Proteomics Facilities

核心——蛋白质相互作用和蛋白质组学设施

• 批准号: 6750903
• 负责人: PAUL M STEMMER
• 金额: $ 18.65万
• 依托单位: WAYNE STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-04-01 至 2009-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6750903
• 关键词: biological signal transduction; biomedical facility; environmental toxicology; fluorescence polarization; fluorescence resonance energy transfer; mass spectrometry; protein binding; protein protein interaction; proteomics; surface plasmon resonance

Description (provided by applicant): The mission of the Protein Interactions and Proteomics (PIP) Facility Core is to enhance research productivity by providing the equipment and expertise necessary for analysis of protein interactions and cellular protein composition. The two components of the PIP Core provide instruments and expertise for identification of proteins and analysis of protein interactions. These two services require different instrumentation, but they both rely on expertise in protein chemistry, separation and analysis. The protein interactions component of the PIP Core provides instrumentation and services for detection of protein binding by Fluorescence Polarization (FP), Surface Plasmon Resonance (SPR), and Fluorescence Resonance Energy Transfer (FRET). These PIP Core technologies, which are used to examine protein interactions, strengthen established research programs of Center investigators with research programs focused on cellular signaling pathways. Invariably, the function of signaling pathways is dependent on the coordinated and regulated interactions between many different proteins. The instruments in the PIP Core produce sensitive, accurate and real time measurements of protein binding events both in vitro and in intact cells. Thus, the protein interactions component of the PIP Core provides a window through which Center investigators can ask questions about protein-protein interactions and the effects of those interactions on signaling pathways and cellular function. The protein identification component of the PIP Core provides access to mass spectrometers for identification of proteins and protein covalent modifications, such as phosphorylation, as well as services for preparation of samples for and running of mass-spec analysis. The PIP Core technologies that are used in protein identification enhance research productivity by providing a clear and easily accessible mechanism for determining the amino acid sequence of unknown proteins that the investigator has either isolated or identified as an important component in a mixture of proteins. The technologies provided by the PIP Core can also be used by Center investigators to identify those proteins in complex mixtures of proteins from body fluids or cells that are candidate biomarkers. Archiving and analysis of data from protein identification technologies requires software systems to maintain the data and expertise in bioinformatics to analyze the data. The EHS Center at Wayne State University has established a facility core for Microarray Analysis and Bioinformatics, which will provide these services to Center investigators. Thus, the protein identification component of the PIP Core provides access to technology for protein identification, protein profiling and biomarker identification.

描述（由申请人提供）：蛋白质相互作用和蛋白质组学（PIP）的使命 Facility Core 旨在通过提供分析蛋白质相互作用和细胞蛋白质组成所需的设备和专业知识来提高研究生产力。 PIP Core 的两个组件为蛋白质鉴定提供仪器和专业知识 和蛋白质相互作用分析。这两项服务需要不同的仪器，但它们都依赖于蛋白质化学、分离和分析方面的专业知识。 PIP Core 的蛋白质相互作用组件提供通过荧光偏振 (FP)、表面等离子共振 (SPR) 和荧光共振能量转移 (FRET) 检测蛋白质结合的仪器和服务。这些用于检查蛋白质相互作用的 PIP Core 技术通过专注于细胞信号通路的研究项目加强了中心研究人员的既定研究项目。信号通路的功能始终依赖于许多不同蛋白质之间的协调和调节的相互作用。 PIP Core 中的仪器可对体外和完整细胞中的蛋白质结合事件进行灵敏、准确和实时的测量。因此，PIP Core 的蛋白质相互作用组件提供了一个窗口，中心研究人员可以通过该窗口提出有关蛋白质-蛋白质相互作用以及这些相互作用对信号通路和细胞功能的影响的问题。 PIP Core 的蛋白质鉴定组件提供对质谱仪的访问，以鉴定蛋白质和蛋白质共价修饰（例如磷酸化），以及用于制备样品和运行质谱分析的服务。用于蛋白质鉴定的 PIP Core 技术通过提供清晰且易于访问的机制来确定研究人员已分离或鉴定为蛋白质混合物中重要成分的未知蛋白质的氨基酸序列，从而提高研究效率。中心研究人员还可以使用 PIP Core 提供的技术来识别体液或细胞中作为候选生物标志物的复杂蛋白质混合物中的蛋白质。蛋白质识别技术数据的归档和分析需要软件系统 维护生物信息学方面的数据和专业知识以分析数据。韦恩州立大学的 EHS 中心已建立了微阵列分析和生物信息学的核心设施，将为中心研究人员提供这些服务。因此，PIP Core 的蛋白质鉴定组件提供了蛋白质鉴定、蛋白质分析和生物标志物鉴定技术。