CORE-- Protein Interactions and Proteomics Facilities

核心——蛋白质相互作用和蛋白质组学设施

• 批准号: 6750903
• 负责人: PAUL M STEMMER
• 金额: $ 18.65万
• 依托单位: WAYNE STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-04-01 至 2009-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6750903
• 关键词: biological signal transduction; biomedical facility; environmental toxicology; fluorescence polarization; fluorescence resonance energy transfer; mass spectrometry; protein binding; protein protein interaction; proteomics; surface plasmon resonance

Description (provided by applicant): The mission of the Protein Interactions and Proteomics (PIP) Facility Core is to enhance research productivity by providing the equipment and expertise necessary for analysis of protein interactions and cellular protein composition. The two components of the PIP Core provide instruments and expertise for identification of proteins and analysis of protein interactions. These two services require different instrumentation, but they both rely on expertise in protein chemistry, separation and analysis. The protein interactions component of the PIP Core provides instrumentation and services for detection of protein binding by Fluorescence Polarization (FP), Surface Plasmon Resonance (SPR), and Fluorescence Resonance Energy Transfer (FRET). These PIP Core technologies, which are used to examine protein interactions, strengthen established research programs of Center investigators with research programs focused on cellular signaling pathways. Invariably, the function of signaling pathways is dependent on the coordinated and regulated interactions between many different proteins. The instruments in the PIP Core produce sensitive, accurate and real time measurements of protein binding events both in vitro and in intact cells. Thus, the protein interactions component of the PIP Core provides a window through which Center investigators can ask questions about protein-protein interactions and the effects of those interactions on signaling pathways and cellular function. The protein identification component of the PIP Core provides access to mass spectrometers for identification of proteins and protein covalent modifications, such as phosphorylation, as well as services for preparation of samples for and running of mass-spec analysis. The PIP Core technologies that are used in protein identification enhance research productivity by providing a clear and easily accessible mechanism for determining the amino acid sequence of unknown proteins that the investigator has either isolated or identified as an important component in a mixture of proteins. The technologies provided by the PIP Core can also be used by Center investigators to identify those proteins in complex mixtures of proteins from body fluids or cells that are candidate biomarkers. Archiving and analysis of data from protein identification technologies requires software systems to maintain the data and expertise in bioinformatics to analyze the data. The EHS Center at Wayne State University has established a facility core for Microarray Analysis and Bioinformatics, which will provide these services to Center investigators. Thus, the protein identification component of the PIP Core provides access to technology for protein identification, protein profiling and biomarker identification.

描述（由申请人提供）：蛋白质相互作用和蛋白质组学的使命（PIP） 设施核心是通过提供分析蛋白质相互作用和细胞蛋白质组成所需的设备和专业知识来提高研究生产率。 PIP核心的两个组成部分提供了识别蛋白质的工具和专业知识 和蛋白质相互作用的分析。这两项服务需要不同的仪器，但它们都依赖于蛋白质化学，分离和分析方面的专业知识。 PIP芯的蛋白质相互作用成分提供了通过荧光极化（FP），表面等离子体共振（SPR）和荧光共振能传递（FRET）检测蛋白质结合的仪器和服务。这些用于检查蛋白质相互作用的PIP核心技术增强了中心研究人员的研究计划，研究计划的研究计划侧重于细胞信号通路。信号通路的功能总是取决于许多不同蛋白质之间的协调和调节相互作用。 PIP芯中的仪器在体外和完整细胞中对蛋白质结合事件产生敏感，准确和实时测量。因此，PIP核心的蛋白质相互作用成分提供了一个窗口，中心研究者可以通过该窗口询问有关蛋白质 - 蛋白质相互作用的问题，以及这些相互作用对信号通路和细胞功能的影响。 PIP核心的蛋白质鉴定成分提供了访问质谱仪的访问，以鉴定蛋白质和蛋白质共价修饰，例如磷酸化，以及为质谱分析和运行样品制备的服务。在蛋白质鉴定中使用的PIP核心技术通过提供了一种清晰易接近的机制来确定研究者在蛋白质混合物中分离或鉴定为重要成分，从而提高了研究生产率。中心研究人员还可以使用PIP核提供的技术来鉴定体液中蛋白质复杂混合物中的这些蛋白质或候选生物标志物的细胞。归档和分析蛋白质识别技术的数据需要软件系统 维护生物信息学方面的数据和专业知识以分析数据。韦恩州立大学的EHS中心已经建立了微阵列分析和生物信息学的设施核心，该设施将为中心调查人员提供这些服务。因此，PIP核心的蛋白质鉴定成分为蛋白质鉴定，蛋白质分析和生物标志物鉴定提供了访问。