Tissue Procurement / Immunohistology

组织采购/免疫组织学

• 批准号: 7116663
• 负责人: RAJIV DHIR
• 金额: $ 4.85万
• 依托单位: UNIVERSITY OF PITTSBURGH AT PITTSBURGH
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-04-01 至 2011-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7116663
• 关键词: clinical research; neoplasm /cancer; tissues; clinical research; neoplasm /cancer; tissues

Core B (Tissue Procurement/lmmunohistology) will operate as a part of the established Tissue and Research Pathology Services (TARPS) Resource at the UPCI. Core B will provide support to all four projects in this Program Project Grant. It will procure, process, and distribute or store specimens from patients with cancer (oral carcinoma and other HNC, melanoma, prostate or renal cell carcinomas) or control individuals, e.g., non-cancer patients undergoing uvulopalatopharyngoplasty ortonsillectomy as controls for HNC cases. Specimens, including tumor biopsies as well as normal tissues adjacent to tumors will be collected from patients or controls, processed by trained personnel in accordance with the established SOP and under rigorous quality control measures. Specimens will be triaged according to the protocol established with the participating investigators and will be accompanied by histopathologic diagnoses and clinical/demographic data. The Core will make available to the PPG investigators snap frozen tissues, frozen sections, conventional paraffin embedded tissues, plastic embedding and sectioning. Core B will provide routine and special pathology services including immunohistochemistry, autoradiography, in-situ hybridization, electron microscopy, quantitative image analysis and morphometry as well as digital imaging and photography. The Core will handle fresh or cryopreserved specimens, microdissect tissues and prepare RNA or DNA as1 needed. The Paraffin Tissue Micro-Array (TMA) facility of the University of Pittsburgh, which is a part of TARPS, will be available to the PPG investigators. Tissue microarrays will also be prepared as needed. Core B will provide annotated clinical information on every specimen used in research from the Core database and function as an "honest broker" to meet HIPAA requirements. Assistance will be available in the preparation of IRB applications for the specimen use and access to associated clinical information.

核心B（组织采购/LMMUNO组织学）将作为已建立组织的一部分作战，并且 UPCI的研究病理服务（TARPS）资源。核心B将为所有四个项目提供支持 在这个计划项目赠款中。它将从患者中采购，处理，分发或存储标本 癌症（口腔癌和其他HNC，黑色素瘤，前列腺或肾细胞癌）或对照个体 例如，正在接受卵疟疾咽成形术的非癌症患者作为HNC病例的对照。 从肿瘤活检以及与肿瘤相邻的正常组织在内的标本将从 患者或对照，由培训的人员根据已建立的SOP及以下处理 严格的质量控制措施。标本将根据与 参与的研究人员将伴随组织病理学诊断和临床/人口统计学 数据。 PPG研究人员将为冷冻组织，冷冻切片，核心提供。 常规的石蜡嵌入组织，塑料嵌入和切片。核心B将提供常规和 特殊病理服务，包括免疫组织化学，放射自显影，原位杂交，电子 显微镜，定量图像分析和形​​态计量学以及数字成像和摄影。这 核心将处理新鲜或冷冻保存的标本，显微解剖组织，并准备RNA或DNA AS1 需要。匹兹堡大学的石蜡组织微阵列（TMA）设施，这是 TARPS将提供给PPG调查人员。组织微阵列也将根据需要准备。核 B将提供有关核心数据库研究中每个标本的注释临床信息 并充当满足HIPAA要求的“诚实经纪人”。协助将在 准备IRB应用程序用于标本的使用和访问相关的临床信息。