TRANSLOCATION OF INHALED ULTRAFINE ELEMENTAL CARBON PARTICLS TO EXTRAPUL TISSUE

吸入的超细元素碳颗粒转移至超薄组织

• 批准号: 7183234
• 负责人: GUNTER OBERDORSTER
• 金额: $ 2.01万
• 依托单位: UNIVERSITY OF CALIF-LAWRNC LVRMR NAT LAB
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-09-01 至 2006-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7183234
• 关键词: analytical method; biomedical equipment resource; clinical research; mass spectrometry; method development; technology /technique development

DESCRIPTION (provided by applicant): The National Resource for Accelerator Mass Spectrometry (AMS) was established in 1999 to enable biomedical researchers to accurately quantify very low levels of radioisotopes while exploring fundamental issues in biology. In this renewal, we will expand our present capabilities by developing methods to study biochemical pathways and cellular processes at the level of the single cell and small groups of cells, which will allow our collaborators to study the effect of aging in yeast. The Resource will also develop methods to quantify endogenous processes, such as protein and DNA oxidation in higher organisms. This will facilitate the study of inflammatory diseases ranging from atherosclerosis to neurodegenerative disorders to cancer. To further these goals, we will develop a sample presentation technology that will enable the analysis of much smaller samples than can be currently processed, as well as techniques for speciation of biomolecules in such samples. Throughout the tenure of the grant we will continue to provide a resource to the research community that will include service to investigators familiar with AMS, training of investigators in the technology and dissemination of the Resource. Towards these goals, our specific aims are to: 1.) Develop protocols for the online combustion of eluents from separatory instruments and couple the products to a gas accepting ion source to increase the sensitivity of AMS. 2.) Increase the value and information content of AMS measurements by quantitating isotope content in isolates of uniformly isotope-labeled systems or by quantitating derivatized biomarkers of modification or function on specific macromolecules. 3.) Provide quantitation of effector and effect in biological systems using multiple isotopic tracers within sampled materials. We will achieve this by developing a robust and higher throughput process for 3H sample preparation and by leveraging against other funded isotope measurements at the Center for Accelerator Mass Spectrometry (CAMS). 4.) Provide high throughput and precise quantitation for collaborative and service users.

描述（由申请人提供）：1999年成立了国家加速器质谱法（AMS）的国家资源，以使生物医学研究人员能够准确地量化非常低的放射性病，同时探索生物学的基本问题。在此续约中，我们将通过开发研究单细胞和小细胞水平的生化途径和细胞过程的方法来扩展当前能力，这将使我们的合作者能够研究酵母中衰老的效果。该资源还将开发量化内源过程的方法，例如较高生物体中的蛋白质和DNA氧化。这将促进从动脉粥样硬化到神经退行性疾病再到癌症的炎症性疾病的研究。为了实现这些目标，我们将开发一种样本演示技术，该技术将能够分析比当前可以处理的要小得多的样本，以及此类样品中生物分子的物种形成的技术。在整个赠款期间，我们将继续为研究社区提供资源，其中包括为熟悉AMS的调查人员提供服务，对技术研究人员的培训以及资源的传播。达到这些目标，我们的具体目标是： 1.）开发从分离仪器的洗脱液的在线燃烧的方案，并将产品与气体接受离子源相结合以提高AM的灵敏度。 2.）通过对均匀同位素标记系统的分离株进行定量的同位素含量，或通过对特定的大分子的修饰或功能进行定量的衍生化生物标志物来增加AMS测量值的价值和信息内容。 3.）使用多个同位素示踪剂对生物系统中的效应子进行定量和效应 在采样材料中。我们将通过开发强大且更高的吞吐量来实现这一目标 3H样品制备的过程，并利用在加速器质谱中心（CAM）的其他资助的同位素测量。 4.）为协作和服务用户提供高吞吐量和精确的定量。