TRANSLOCATION OF INHALED ULTRAFINE ELEMENTAL CARBON PARTICLS TO EXTRAPUL TISSUE

吸入的超细元素碳颗粒转移至超薄组织

• 批准号: 7602410
• 负责人: GUNTER OBERDORSTER
• 金额: $ 2.1万
• 依托单位: UNIVERSITY OF CALIF-LAWRNC LVRMR NAT LAB
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-09-01 至 2008-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7602410
• 关键词: Adverse effects; Air; Animals; Brain; Breathing; Caliber; Carbon; Cerebellum; Cerebrum; Chemicals; Clinical Research; Collaborations; Computer Retrieval of Information on Scientific Projects Database; Deposition; Epidemiologic Studies; Funding; Gases; Grant; Heart; In Vitro; Injection of therapeutic agent; Institution; Kidney; Label; Liver; Long-Term Effects; Lung; Medical; Methods; Nanotechnology; Organ; Particulate; Pollution; Range; Rattus; Research; Research Personnel; Resources; Respiratory System; Site; Source; Spleen; Tissues; Ultrafine; United States National Institutes of Health; accelerator mass spectrometry; anthropogenesis; base; particle; size; ultrafine particle

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Ultrafine particles (particles 100 nm in diameter) are ubiquitous in the ambient air from anthropogenic and natural sources (e.g., internal combustion engine emissions, gas to particle conversions). The new field of nanotechnology in industrial and medical use adds an additional component. Inhalation of ultrafine particles is known to cause adverse effects in pulmonary and extrapulmonary tissues. Based on our earlier studies with ultrafine 13C elemental carbon particles we hypothesize that such particles can translocate from sites of deposition to extrapulmonary organs such as the liver and the brain. Due to the inherent problem of 13C background levels in tissues for assessing significant increases in added tissue carbon a more sensitive method is needed. We propose to generate ultrafine elemental carbon particles labeled with 14C to which rats will be exposed by inhalation, gavage and i.v. injection to these particles. The size of the ultrafine 14C particles ranges from ~10 nm to ~80 nm. Rats will be sacrificed at different timepoints after exposure and lungs, liver, heart, kidney, spleen and brain sections (olfactory; cerebrum; cerebellum) excised and prepared for 14C accelerator mass spectrometry. These studies are part of an EPA-funded Ultrafine Particle Center at Rochester. Studies performed by Center investigators focus on physico-chemical characterization of ambient UFP; epidemiological studies; controlled clinical studies; toxicological animal studies; and mechanistic in vitro studies. The proposed research in collaboration with LLNL will have great significance to elucidate the fate of inhaled ultrafine particles after their deposition in the respiratory tract. If significant amounts of ultrafine particles are translocated to sensitive sites such as the heart and the brain, it may explain both short-term and long-term effects of ambient particulate pollution.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 超铁颗粒（直径为100 nm的颗粒）在人为和天然来源的环境空气中无处不在（例如，内燃烧发动机排放，气体到粒子转换）。 纳米技术在工业和医疗用途中的新领域增加了另一个组成部分。 已知吸入超细颗粒会在肺和肺外组织中引起不良反应。 基于我们对Ultrafine 13C元素碳颗粒的早期研究，我们假设这些颗粒可以从沉积部位转移到肺外器官，例如肝脏和大脑。 由于组织中13c背景水平的固有问题用于评估添加的组织碳的显着增加，因此需要更敏感的方法。 我们建议生成用14C标记的超细元素碳颗粒，并通过吸入，饲料和静脉注射大鼠。注入这些颗粒。 Ultrafine 14C颗粒的大小范围从〜10 nm至〜80 nm。 暴露和肺部，肝脏，心脏，肾脏，脾脏和脑切片（嗅觉；大脑；小脑）在暴露和肺部，肝脏，心脏，肾脏，脾脏和脑部后，将在不同的时间点处死大鼠，并准备14C加速器质谱。 这些研究是罗切斯特EPA资助的超细粒子中心的一部分。 中心研究人员对环境UFP的物理化学表征进行的研究；流行病学研究；受控临床研究；毒理学研究；和体外研究机械。 拟议的研究与LLNL合作将具有极大的意义，即在沉积在呼吸道中后阐明吸入的超细颗粒的命运。 如果将大量的超细颗粒转移到敏感部位（例如心脏和大脑），则可以解释环境颗粒污染的短期和长期影响。