SHIVERER MOUSE/EAE BIRN COLLABORATION

SHIVERER MOUSE/EAE BRN 合作

• 批准号: 7181545
• 负责人: ALAN MACKENZIE-GRAHAM
• 金额: $ 0.53万
• 依托单位: DUKE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-07-01 至 2006-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7181545
• 关键词: bioimaging /biomedical imaging; biomedical resource; imaging /visualization /scanning; microscopy; technology /technique development

DESCRIPTION (provided by applicant): The enormous growth in the use of small animals for basic biomedical research has created a compelling need for new approaches to structural and functional phenotyping in the rodent. We propose continuation of the Duke Integrated Center for In Vivo Microscopy, an NIH/NCRR National Resource with an explicit focus on meeting this need. We propose four integrated cores that will provide researchers from around the world new image-based methods for structural and functional phenotyping using magnetic resonance microscopy (MRM). Core A: We will develop functional microscopy in the rodent to support cardiac motion analysis, pulmonary perfusion using arterial spin tagging and pulmonary ventilation using hypcrpolarized gas imaging. Core B: We will develop more efficient encoding strategies to increase our throughput for MR Histology by at least 500%. We will develop methods for actively staining fixed tissues for 3D MR histology. We will develop methods to measure regional neurodegeneration in the mouse brain. Core C: We will develop a new generation of technologies for physiologic support and monitoring of small animals permitting mutlimodality imaging (MRM. MicroPET, microCT, etc.) and image registration and long term studies on animals with compromised immunologic systems. Core D: We will develop visual informatics tools to permit effective archive, access, and analysis of very large multidimensional image sets from multiple modalities. The technologies will support a total of 16 collaborators anxious to use small animal imaging to extend the horizons of topics ranging from cancer to neurobiology. An aggressive education and dissemination program is included that will include undergraduate and graduate students, as well as post-doctoral fellows.

描述（由申请人提供）： 小动物用于基本生物医学研究的巨大生长使人们对啮齿动物的结构和功能表型的新方法产生了迫切的需求。我们建议延续Duke Integrated体内显微镜中心，这是NIH/NCRR国家资源，明确着重于满足这一需求。我们提出了四个集成的核心，这些核心将为世界各地的研究人员提供新的基于磁共振显微镜（MRM）的基于图像和功能表型的方法。核心A：我们将在啮齿动物中发展功能显微镜，以支持心脏运动分析，使用动脉自旋标记的肺灌注以及使用催眠气体成像的肺部通风。核心B：我们将制定更有效的编码策略，以将MR组织学的吞吐量提高至少500％。我们将开发用于主动染色3D MR组织学的固定组织的方法。我们将开发测量小鼠脑中区域神经退行性的方法。核心C：我们将开发新一代的技术，用于生理支持和监测允许MutLimodality成像的小动物（MRM。Icropet，Microct等）以及图像注册以及对具有受损免疫系统的动物的长期研究。核心D：我们将开发视觉信息学工具，以允许对多种模式的非常大的多维图像集进行有效的存档，访问和分析。这些技术将支持16个急切使用小动物成像的合作者，以扩展从癌症到神经生物学等主题的视野。包括一项积极的教育和传播计划，其中包括本科生和研究生以及博士后研究员。