SHIVERER MOUSE/EAE BIRN COLLABORATION

SHIVERER MOUSE/EAE BRN 合作

• 批准号: 7956862
• 负责人: ALAN MACKENZIE-GRAHAM
• 金额: $ 0.55万
• 依托单位: DUKE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-07-01 至 2010-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7956862
• 关键词: Animals; Collaborations; Computer Retrieval of Information on Scientific Projects Database; Disease; Funding; Grant; Histologic; Histology; Image; Institution; Microscopy; Mus; Mutant Strains Mice; Myelin; Protocols documentation; Research; Research Personnel; Resources; Source; Staining method; Stains; Structure; United States National Institutes of Health; dysmyelination; in vivo

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This project aims to correlate dysmyelination using MRM and histology. Comparisons have been made between cytoarchitecture and MRM, but not for myeloarchitecture and MRM. We will compare histological stains for myelin with images from different MRM protocols in the same animal in order to visualize dysmyelination in the shiverer mouse. Additionally, comparisons will be made between neuroanatomical structures in both the wild-type and shiverer mutant mice to see if dysmyelination causes changes in the underlying antomy, looking at both cytoarchitecture and myeloarchitecture. We are looking for MRM correlates of histologically observed dysmyelination. Preliminary results show that dysmyelination can be visualized using MRM. This would have far-reaching implications for following dysmyelinating diseases in vivo using MRM.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 该项目的目的是使用MRM和组织学相关联合会。 在细胞结构和MRM之间进行了比较，但没有用于骨髓结构和MRM。我们将将髓磷脂的组织学染色与同一动物中不同MRM方案的图像进行比较，以可视化Shiverer小鼠中的失调症。此外，将在野生型和颤抖的突变体小鼠中的神经解剖结构进行比较，以查看肾上腺素是否会导致潜在的抗抑制作用的变化，从而查看细胞结构和骨髓结构。 我们正在寻找组织学观察到的炎症性的MRM相关性。初步结果表明，可以使用MRM可视化障碍。 这对于使用MRM在体内遵循肿瘤疾病的疾病会具有深远的影响。