High Resolution Mapping of Pain in the Dorsal Horn

背角疼痛的高分辨率测绘

• 批准号: 6869911
• 负责人: Andrew Mark Strassman
• 金额: $ 19.66万
• 依托单位: BETH ISRAEL DEACONESS MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-01-15 至 2006-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6869911
• 关键词: bioimaging /biomedical imaging; biomedical resource; brain imaging /visualization /scanning; brain mapping; dorsal horn; laboratory rat; lasers; neuroanatomy; neurons; pain; technology /technique development

DESCRIPTION (provided by applicant): In recent years, pain research has made revolutionary advances at levels ranging from genes to behavior. However, one basic level of organization that has proved particularly resistant to analysis is the intrinsic circuitry underlying pain transmission and modulation within the first central relay area, the spinal/medullary dorsal horn. Recently a novel technique was developed that has made it possible for the first time to dissect the fine organization of the synaptic connectivity of closely spaced neural elements with extraordinary spatial resolution. This technique, scanning laser photostimulation, would be ideal for revealing for the first time the intrinsic organization of the dorsal horn circuitry involved in pain. However, the initial setting up of this technique is fairly expensive and time-consuming, and its use has so far been almost entirely restricted to cerebral cortex, primarily in the visual system. I request 2 years of support to set up and collect initial data with scanning laser photostimulation to study pain (nociceptive) circuitry in the dorsal horn. The application of this technique to the pain system will open up to analysis for the first time an entire field of investigation into plasticity of dorsal horn circuitry and allow the resolution of questions that have been debated since the start of the modern era of pain research and the proposal of the gate-control theory of pain modulation.

描述（由申请人提供）：近年来，疼痛研究已在基因到行为的水平上取得了革命性的进步。但是，事实证明，一个基本的组织水平是对分析尤其抗药性的，这是第一个中央继电器区域内的疼痛传播和调节的固有电路，即脊柱/髓质背角。最近，开发了一种新颖的技术，该技术首次可以通过非凡的空间分辨率剖析紧密间隔神经元素的突触连通性的良好组织。该技术，即扫描激光光刺激，是首次揭示背角回路的内在组织所涉及的疼痛的理想选择。但是，该技术的初始设置相当昂贵且耗时，并且到目前为止，它的使用几乎完全仅限于大脑皮层，主要是在视觉系统中。我要求用扫描激光光刺激来设置和收集初始数据2年，以研究背角中的疼痛（伤害感受）电路。该技术在疼痛系统中的应用将首次开放分析背角回路可塑性的整个研究领域，并允许自现代疼痛研究开始以来一直在辩论的问题和疼痛模式的栅极控制理论提出的提议。