Triple Quadrupole Mass Spectrometer With HPLC System

带 HPLC 系统的三重四极杆质谱仪

• 批准号: 6877217
• 负责人: Stanley L Hazen
• 金额: $ 50万
• 依托单位: CLEVELAND CLINIC LERNER COM-CWRU
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-05-01 至 2006-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6877217
• 关键词: biomarker; biomedical equipment purchase; high performance liquid chromatography; liquid chromatography mass spectrometry; protein quantitation /detection; protein sequence

DESCRIPTION (provided by applicant): The purpose of this proposal is to seek funds for the purchase of a triple quadruple mass spectrometry system with multiplex HPLC interface. This instrument will expand mass spectrometry capabilities within the Northeastern Ohio region, advancing research programs within the Cleveland Clinic Foundation (CCF) and its partner institution, Case Western Reserve University (CWRU). Major users all have NIH-funded research grants, including new Program Project and SCCOR programs, which have projects that are currently conducted using mass spectrometry. Because of full utilization of available instrumentation in the region, many investigators now have to schedule time months in advance to obtain access to this technology. As detailed in the application, supported projects primarily include biomarker discovery and clinical/translational studies. The requested mass spectrometer provides the high through-put capabilities that will assure timely access to this type of instrumentation, while not sacrificing versatility, sensitivity and specificity required for these projects. This analytical activity will be conducted within our existing Mass Spectrometry Core, which provides support to investigators at both CCF and CWRU by performing structural elucidation studies, biomarker quantification, protein sequencing/identification, and related services. Mass spectrometry is increasingly being used as an analytical tool by CCF and CWRU investigators, with demand greatly outpacing availability, resulting in significant delays in access, and hampered productivity. The acquisition of the requested mass spectrometer is essential to alleviate the backlog of samples waiting to be analyzed. CCF has committed substantial resources to the development of mass spectrometry in the region, including custom renovation of laboratory space for Mass Spectrometry Core, personnel to oversee its operation, and a fiscal plan to insure its operation and maintenance for the long-term.

描述（由申请人提供）：本提案的目的是寻求资金购买具有多重 HPLC 接口的三重四极杆质谱系统。该仪器将扩大俄亥俄州东北部地区的质谱分析能力，推进克利夫兰诊所基金会 (CCF) 及其合作机构凯斯西储大学 (CWRU) 内的研究项目。主要用户都拥有 NIH 资助的研究经费，包括新计划项目和 SCCOR 计划，其中有目前使用质谱法进行的项目。由于充分利用了该地区现有的仪器，许多研究人员现在必须提前几个月安排时间才能获得这项技术。正如申请中详细说明的，支持的项目主要包括生物标志物发现和临床/转化研究。所需的质谱仪提供高通量能力，确保及时访问此类仪器，同时不牺牲这些项目所需的多功能性、灵敏度和特异性。这项分析活动将在我们现有的质谱核心内进行，该核心通过进行结构阐明研究、生物标志物定量、蛋白质测序/鉴定和相关服务，为 CCF 和 CWRU 的研究人员提供支持。质谱法越来越多地被 CCF 和 CWRU 研究人员用作分析工具，但需求远远超过了可用性，导致访问严重延迟，并影响了生产力。获取所需的质谱仪对于缓解等待分析的样品积压至关重要。 CCF 已投入大量资源用于该地区质谱技术的发展，包括质谱核心实验室空间的定制改造、监督其运营的人员以及确保其长期运营和维护的财政计划。