LTQ Linear Ion Trap Mass Spectrometer System

LTQ 线性离子阱质谱仪系统

• 批准号: 6879828
• 负责人: SUSAN T WEINTRAUB
• 金额: $ 49.72万
• 依托单位: UNIVERSITY OF TEXAS HLTH SCIENCE CENTER
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-04-01 至 2006-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6879828
• 关键词: biomedical equipment purchase; computers; high performance liquid chromatography; mass spectrometry; phosphopeptides; protein quantitation /detection

DESCRIPTION (provided by applicant): Funds are requested to purchase a Thermo Finnigan LTQ linear ion trap mass spectrometer, accompanying micro HPLC system and computer cluster for the Institutional Mass Spectrometry Laboratory (MSL) of the University of Texas Health Science Center at San Antonio, under the direction of Dr. Susan Weintraub, who has over 30 years experience in biomedical mass spectrometry. At the present time, capillary HPLC-ESI/ MS/MS analyses are conducted on a Thermo Finnigan LCQ-Classic that was installed in 1997, and MALDI-TOF mass spectra are acquired on an Applied Biosystems Voyager-DE STR. These instruments have been extraordinarily powerful tools for identification and characterization of proteins and peptides for a large number of investigators. The 2003 user group consisted of 36 individual faculty members from 14 departments at UTHSCSA in addition to a number of researchers from other institutions in Texas and elsewhere in the U.S. Several technological limitations of the LCQ-Classic along with concerns about its operating lifetime prompted efforts to seek funding for a new instrument for the MSL. Regarding the performance of the instrument, improvements in detection limits, scan speed and sample through-put would benefit a large proportion of the user group. Advantages of the LTQ related to routine maintenance would enhance efficiency of day-to-day usage. Dr. Weintraub evaluated the LTQ during a trip to San Jose in February 2004 at which time she analyzed representative samples prepared in her laboratory. The results clearly demonstrated the significant benefits to the user group of the MSL that will result from acquisition of an LTQ in the following areas: (1) identification of low levels of protein in a gel; (2) identification of a low level of protein in a complex mixture in a gel; and (3) detection of a phosphopeptide at low stoichiometry. Project descriptions for 9 major users, 4 minor users and 3 future users are presented.

描述（由申请人提供）：需要资金购买 Thermo Finnigan LTQ 线性离子阱质谱仪、配套的微型 HPLC 系统和计算机集群，用于圣安东尼奥德克萨斯大学健康科学中心的机构质谱实验室 (MSL)，在 Susan Weintraub 博士的指导下，她在生物医学质谱领域拥有 30 多年的经验。目前，毛细管 HPLC-ESI/MS/MS 分析是在 1997 年安装的 Thermo Finnigan LCQ-Classic 上进行的，MALDI-TOF 质谱是在 Applied Biosystems Voyager-DE STR 上采集的。这些仪器对于大量研究人员来说是鉴定和表征蛋白质和肽的极其强大的工具。 2003 年的用户组由来自 UTHSCSA 14 个系的 36 名教职人员以及来自德克萨斯州和美国其他地区其他机构的一些研究人员组成。LCQ-Classic 的一些技术限制以及对其运行寿命的担忧促使人们努力为 MSL 的新工具寻求资金。就仪器性能而言，检测限、扫描速度和样品吞吐量的改进将使大部分用户群体受益。 LTQ 与日常维护相关的优势将提高日常使用的效率。 Weintraub 博士在 2004 年 2 月前往圣何塞期间评估了 LTQ，当时她分析了实验室制备的代表性样品。结果清楚地证明了获得 LTQ 在以下领域将为 MSL 用户组带来的显着好处：(1) 鉴定凝胶中的低水平蛋白质； (2) 鉴定凝胶中复杂混合物中的低水平蛋白质； (3)低化学计量的磷酸肽的检测。提供了 9 个主要用户、4 个次要用户和 3 个未来用户的项目描述。

项目成果

Vaccine-mediated immune responses to experimental pulmonary Cryptococcus gattii infection in mice.

• DOI: 10.1371/journal.pone.0104316
• 发表时间: 2014
• 期刊: PloS one
• 影响因子: 3.7
• 作者: Chaturvedi AK;Hameed RS;Wozniak KL;Hole CR;Leopold Wager CM;Weintraub ST;Lopez-Ribot JL;Wormley FL Jr
• 通讯作者: Wormley FL Jr