MASS SPECTROMETRY SHARED RESOURCE

质谱共享资源

基本信息

批准号：
7944762
负责人：
SUSAN T WEINTRAUB
金额：
$ 2.47万
依托单位：
UNIVERSITY OF TEXAS HLTH SCIENCE CENTER
依托单位国家：
美国
项目类别：
财政年份：
2009
资助国家：
美国
起止时间：
2009-08-03 至 2012-07-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7944762
关键词：
Amino Acids Anus Biological Cancer Center Categories Cells Chemicals Chromatography Citric Acid Cycle Communities Complex Mixtures Computer software Devices Diamond Digestion Electrons Excision Exhibits Fluorescent Dyes Gases Gel High Pressure Liquid Chromatography Housing Image Analysis Individual Ions Label Lasers Length Licensing Lipids Mass Spectrum Analysis Measures Methods Molecular Weight Nonlinear Dynamics Nucleic Acid Databases Peptides Phase Phosphoproteins Phosphorylation Pro-Q aerosol foam Protein Analysis Proteins Proteome Proteomics Relative (related person)Research Research Personnel Resource Sharing Running Sampling Science Sequence Analysis Services Site Slice Solid Solutions Source Spectrum Analysis Spottings Stable Isotope Labeling Staging Staining method Stains Subcellular Fractions System Therapeutic Studies Time Tissues Two-Dimensional Gel Electrophoresis base cancer therapy comparison group cyanine dye 5 gel electrophoresis instrument ionization mass spectrometer mass spectrometry shared resource member molecular mass polyacrylamide gels programs protein expression quantum research study scaffold selected ion monitoring small molecule stable isotope

项目摘要

The Mass Spectrometry (MASS SPECTROSCOPY) Shared Resource offers a wide range of analytical capabilities to support the needs of Cancer Center investigators and other researchers at UTHSCSA and in the nearby community. Mass spectrometry instrumentafion in the MASS SPECTROSCOPY Shared Resource is as follows: Thermo Fisher LTQ ion trap tandem mass spectrometer used in conjunction with an Eksigent NanoLC micro HPLC system to perform nanospray HPLC/MASS SPECTROSCOPY" analyses; Thermo Fisher Quantum-AM triple quadrupole mass spectrometer used with a Michrom BioResources Paradigm MASS SPECTR0SC0PY4 micro HPLC system; Thermo Fisher LCQ ion trap mass spectrometer used in conjunction with a Michrom BioResources Paradigm MASS SPECTR0SC0PY4 micro HPLC system; Applied BiosysteMass Spectroscopy Voyager DE-STR matrixassisted laser desorpfion ionization fime-of-flight mass spectrometer (MALDI-TOF/MASS SPECTROSCOPY); Thermo Fisher DSQ quadrupole mass spectrometer which is able to perform GC/MASS SPECTROSCOPY and direct probe analyses using electron impact and chemical ionizafion with positive and negafive ion detecfion. In addition to the mass spectrometers, devices that greafiy facilitate protein characterization are available in the facility, including an imager, densitometer, gel spotcutter and PDQuest gel analysis software (all from Bio-Rad) and SameSpots gel analysis software from Nonlinear Dynamics. The major protein analysis services provided include gel electrophoresis, molecular mass determinafion, protein idenfificafion from solufion or polyacrylamide gel, sequence characterizafion, and elucidation of sites of post-translafional modificafion. Detecfion limits in the sub-picomole range are roufinely obtained, making it possible to characterize proteins and pepfides isolated from biological samples. Quantitative analysis of lipids and intermediary metabolites by GC/MASS SPECTROSCOPY is also performed on a regular basis.

质谱（MASS SPECTROSCOPY）共享资源提供了广泛的分析支持癌症中心研究人员以及 UTHSCSA 和其他研究人员的需求的能力附近的社区。质谱共享中的质谱仪器资源如下：Thermo Fisher LTQ离子阱串联质谱仪配合使用 Eksigent NanoLC 微型 HPLC 系统，用于执行纳喷雾 HPLC/质谱分析" 分析； Thermo Fisher Quantum-AM 三重四极杆质谱仪与 Michrom 配合使用 BioResources Paradigm MASS SPECTR0SC0PY4 微量 HPLC 系统； Thermo Fisher LCQ 离子阱与 Michrom BioResources Paradigm MASS 结合使用的质谱仪 SPECTR0SC0PY4 微量高效液相色谱系统； Applied Biosyste质谱 Voyager DE-STR 基质辅助激光解吸电离飞行时间质谱仪 (MALDI-TOF/MASS 光谱学）； Thermo Fisher DSQ 四极杆质谱仪能够执行使用电子轰击和化学电离进行 GC/MASS 光谱和直接探针分析正负离子检测。除了质谱仪之外，还可以极大地促进该设施提供蛋白质表征，包括成像仪、密度计、凝胶切点仪和 PDQuest 凝胶分析软件（全部来自 Bio-Rad）和来自 Nonlinear 的 SameSpots 凝胶分析软件动力学。提供的主要蛋白质分析服务包括凝胶电泳、分子量测定、溶液或聚丙烯酰胺凝胶中的蛋白质鉴定、序列表征以及阐明翻译后修饰位点。亚皮摩尔范围内的检测限为常规获得，使得能够表征从生物中分离的蛋白质和肽样品。通过 GC/MASS 光谱对脂质和中间代谢物进行定量分析也定期进行。