Zeiss Axiovert 200-M for Time-Lapse Microscopy

用于延时显微镜的 Zeiss Axiovert 200-M

• 批准号: 6877463
• 负责人: Peter N Devreotes
• 金额: $ 17.36万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-06-01 至 2006-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6877463
• 关键词: biomedical equipment purchase; charge coupled device camera; microscopy

DESCRIPTION (provided by applicant): This is an application for a Zeiss inverted microscope with precision motorized stage and sensitive CCD camera for time-lapse, live-cell imaging. The microscope will be used as a dedicated station for constant-focus time-lapse imaging with DIG and fluorescence optics. The precision AS) motorized piezo microscope stage and the Zeiss environmental control system will allow us to image cells at constant focus under physiological conditions for hours or days at a time. This stage will also be used to prepare time-lapse sequences of cells at different locations during the same imaging session. The sensitive Photometrics Cascade CCD camera will significantly reduce photobleaching and maintain cell viability. This application is based on a critical need to monitor cell responses over time by time-lapse recording and will serve a large NIH-funded community.

描述（由申请人提供）：这是用于具有精确机动阶段和灵敏的CCD摄像头的Zeiss倒置显微镜的应用程序，可用于延时，实时成像。显微镜将用作使用DIC和荧光光学器件的恒定聚焦时置成像的专用站。精确度为）电动压电显微镜阶段和Zeiss环境控制系统将使我们能够一次在生理条件下持续焦点图像小时或几天。该阶段还将用于在同一成像过程中在不同位置的不同位置准备延时序列。敏感的光电级联CCD摄像头将显着降低光漂白并维持细胞活力。该应用程序是基于通过延时记录来监测细胞响应的迫切需要，并将为大型NIH资助的社区提供服务。