Examination of Odorant Receptors in Anopheles gambiae

冈比亚按蚊气味受体的检测

• 批准号: 6838786
• 负责人: LAURENCE J ZWIEBEL
• 金额: $ 45.22万
• 依托单位: VANDERBILT UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-07-01 至 2007-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6838786
• 关键词: Examination; Odorant; Receptors; Anopheles; gambiae

EXCEED THE SPACE PROVIDED. Chemosensory responses are critical components that direct several essential behaviors of insects that are vectors for pathogens responsible for many important human diseases. In particular, olfaction plays a major role in host seeking and selection behavior of blood-feeding female mosquitoes and as such, constitutes a critical component of the mosquito's ability to transmit diseases such as malaria, dengue, yellow fever and West Nile Nile virus encephalitis. Within this context, I have undertaken a molecular examination of several elements of the olfactory signal transduction cascade in the principal African malaria vector mosquito Anopheles gambiae sensu stricto where a significant preference for human hosts (anthropophily) underlies its inherent ability to transmit human malaria. An increased understanding of olfactory mechanisms and their underlying chemical cues in this system may provide insight in the processes of insect behavioral responses in general and vector disease transmission in particular. Moreover, this study would likely be instrumental in the development of novel mosquito control strategies targeted against this broad family of disease vectors as well as other insects that pose considerable medical and economic threats through their abilty to transmit disease and to act as agricultural pests. The objectives of this proposal complements ongoing studies in the laboratory of olfactory arrestins in A. gambiae and focus on an examination of a family of candidate odorant receptor proteins in A. gambiae (AgORs) that has recently been identified by this laboratory. Thus far, we have identified 44 candidate AgORs and have performed an initial characterization of a small subset of this gene family. While the exact number of potential AgORs remain to be established in A. gambiae, we proposed to begin a systematic analysis of this gene family by completing several studies focusing on their expression in the mosquito. Intially, in order to establish a strategic plan for future studies, we will employ a variety of molecular approaches to identify AgOR family members that exhibt sex-specific expression patterns. These AgORs will form our primary focus group for subsequent characterization of the developmental, spatial and temporal expression pattems of AgORs. In addition, in a extension of initial studies that specifically targets AgOR's role in the olfactory processes that underlie disease transmission, will we propose to conduct a series of detailed experiments to examine the temporal kinetics of AgOR expression in response to blood feeding PERFORMANCE SITE ========================================Section End===========================================

超出所提供的空间。 化学感应反应是指导昆虫几种基本行为的关键组成部分，而昆虫是导致许多重要人类疾病的病原体的载体。特别是，嗅觉在吸血雌性蚊子的宿主寻找和选择行为中起着重要作用，因此，嗅觉构成了蚊子传播疟疾、登革热、黄热病和西尼罗河尼罗病毒脑炎等疾病的能力的关键组成部分。在此背景下，我对非洲主要疟疾媒介冈比亚按蚊（Anopheles gambiae sensu stricto）嗅觉信号转导级联的几个要素进行了分子检查，其中对人类宿主的显着偏好（拟人性）是其传播人类疟疾的固有能力的基础。加深对嗅觉机制及其在该系统中的潜在化学线索的了解，可以深入了解昆虫的一般行为反应过程，特别是媒介疾病传播的过程。此外，这项研究可能有助于制定针对这一广泛的疾病媒介家族以及其他昆虫的新型蚊子控制策略，这些昆虫通过传播疾病和充当农业害虫的能力而构成相当大的医疗和经济威胁。该提案的目的是补充冈比亚曲霉嗅觉抑制蛋白实验室正在进行的研究，并重点检查该实验室最近鉴定的冈比亚曲霉嗅觉受体蛋白（AgOR）家族的候选气味受体蛋白。到目前为止，我们已经鉴定了 44 个候选 AgOR，并对这个基因家族的一小部分进行了初步表征。虽然冈比亚蚊中潜在 AgOR 的确切数量仍有待确定，但我们建议通过完成几项关注其在蚊子中表达的研究来开始对该基因家族进行系统分析。首先，为了制定未来研究的战略计划，我们将采用多种分子方法来鉴定表现出性别特异性表达模式的 AgOR 家族成员。这些 AgOR 将构成我们的主要焦点小组，用于后续表征 AgOR 的发育、空间和时间表达模式。此外，在专门针对 AgOR 在疾病传播的嗅觉过程中的作用的初步研究的延伸中，我们是否建议进行一系列详细的实验来检查 AgOR 表达响应血液喂养的时间动力学========================================章节结束========== ===================================