Investigating the Role of Gr23 Gene in the Mating Capacity of Malaria Vector Mosquito Anopheles coluzzii

Gr23基因在疟疾媒介蚊子按蚊交配能力中的作用研究

基本信息

批准号：
10425425
负责人：
LAURENCE J ZWIEBEL
金额：
$ 7.93万
依托单位：
VANDERBILT UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2021
资助国家：
美国
起止时间：
2021-06-09 至 2023-05-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10425425
关键词：
Address Adopted Aedes Afferent Neurons Alleles Animals Anopheles Genus Arthropods Behavior Blood Breeding Candidate Disease Gene Carbon Dioxide Cessation of life Chemicals Code Complex Cues Culicidae Defect Dengue Detection Diptera Environment Exhibits Failure Female Filarial Elephantiases Gene Targeting Genes Human Hydrocarbons In Vitro Japanese Encephalitis Leg Malaria Maxilla Mediating Membrane Molecular Molecular Target Mosquito Control Neurons Odorant Receptors Odors Parasites Partner in relationship Pattern Persons Pheromone Plasmodium falciparum Play Process Public Health Reproduction Research Proposals Role Semiochemicals System Testing Triad Acrylic Resin West Nile Encephalitis Work Xenopus oocyte Yellow Fever Zika Virus appendage base carbon dioxide receptor feeding in vivo labia male mating behavior mutant novel olfactory sensory neurons pathogen receptor reproductive response transcriptomics vector vector mosquito

项目摘要

Project Summary As one of the most important vectors for the malaria parasite Plasmodium falciparum, which infected approximately 228 million people worldwide in 2018 and accounted for more than 400,000 deaths (WHO, 2019), the An. coluzzii mosquito has long been a formidable concern to public health. These mosquitoes rely heavily on their chemosensory system to detect chemical cues involved in the mating process, including aggregation pheromones and cuticular hydrocarbons. Swarm aggregation pheromones increase the possibility of encountering female mosquitoes while cuticular hydrocarbons help male mosquitoes recognize conspecific females. A recent finding of ours indicated that not only were Gr23-null mutants insensitive to carbon dioxide stimulation, they also appeared to have some mating defect, as they failed to self-breed. Previous transcriptomic studies in Aedes mosquitoes indicated that Gr23 is expressed in CO2-sensitive neurons in the maxillary palps, but presents the highest expression among all gustatory receptors in the antennae and other chemosensory appendages, which suggests additional roles of Gr23 in chemosensation beyond CO2 reception. Therefore, the aim of this research proposal is to determine the role of Gr23 in the mating capacity of An. coluzzii. Here, we hypothesize that the observed self-breeding defect of Gr23 mutants is due to their inability to detect mating-related chemical cues, and that the Gr23 receptor plays a critical role in this chemosensory process. Accordingly, the fundamental questions I propose to address are: (1) what is the expression pattern of Gr23 in the chemosensory neurons of the mating-related appendages in An. coluzzii? (2) What is the molecular function of Gr23 in detecting the mating-related semiochemicals? In addressing these questions, this work will further our understanding of the role of Gr23 in the mosquito's odor-mediated mating process, which would have great potential as a novel molecular target in mosquito control.

项目概要作为疟原虫恶性疟原虫最重要的传播媒介之一， 2018 年，全球约有 2.28 亿人死亡，死亡人数超过 40 万（世界卫生组织， 2019），安。长期以来，coluzzii 蚊子一直是公众健康的一个严重问题。这些蚊子依靠很大程度上依靠化学感应系统来检测交配过程中涉及的化学线索，包括聚集信息素和角质层碳氢化合物。群体聚集信息素增加了可能性遇到雌性蚊子的概率，而表皮碳氢化合物帮助雄性蚊子识别同种蚊子女性。我们最近的一项发现表明，Gr23 缺失突变体不仅对二氧化碳不敏感刺激下，它们似乎也有一些交配缺陷，因为它们无法自我繁殖。以前的伊蚊的转录组研究表明 Gr23 在 CO2 敏感神经元中表达上颌触须，但在触角和其他部位的所有味觉感受器中表达最高化学感应附属物，这表明 Gr23 在化学感应中除了 CO2 之外还有其他作用接待。因此，本研究计划的目的是确定 Gr23 在交配能力中的作用安的。科鲁兹。在这里，我们假设观察到的 Gr23 突变体的自交缺陷是由于它们的无法检测与交配相关的化学信号，而 Gr23 受体在其中发挥着关键作用化学感应过程。因此，我建议解决的基本问题是：（1）什么是 Gr23 在 An 交配相关附肢化学感应神经元中的表达模式。科鲁兹？ (2) Gr23 在检测交配相关化学信息素方面的分子功能是什么？在解决这些问题时问题，这项工作将进一步了解 Gr23 在蚊子气味介导的交配中的作用过程，这将具有作为蚊子控制的新型分子靶标的巨大潜力。