Retinoic acid in motor neuron specification

运动神经元规范中的视黄酸

• 批准号: 7312771
• 负责人: EDUARDO ROSA-MOLINAR
• 金额: $ 25.49万
• 依托单位: UNIVERSITY OF PUERTO RICO MED SCIENCES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7312771
• 关键词: Puerto Rico; alternatives to animals in research; cell biology; cell cell interaction; cell differentiation; cell migration; cerebral ventricles; cooperative study; developmental neurobiology; electroporation; enzyme activity; fish; glia; immunocytochemistry; minority institution research support; molecular /cellular imaging; molecular probes; motor neurons; neurogenesis; nonmammalian vertebrate embryology; retinoate; spinal cord; vitamin metabolism; zebrafish

The proposed project of Drs. Eduardo Rosa-Molinar, the University of Puerto Rico-Rio Piedras (UPR-RP), and Scott E. Fraser of the California Institute of Technology (CalTech) will result in a greater understanding of the cellular interactions that guide the development of the spinal cord. In particular, the experiments will establish the phenotypes of the cells that contain the enzymes synthesizing and degrading retinoic acid (RA) and elucidate their role in guiding the genesis, migration, and fate of germinal cells in the ependyma of the embryonic spinal cord, specifically, secondary motor neurons. The experimental plan builds upon previous National Science Foundation funded work performed in the Rosa-Molinar laboratory. That work established both the RA metabolism and the functional neuroanatomy of the spinal cord in the developing and mature Western Mosquitofish, Gambusia affinis affinis (hereafter "mosquitofish"). The proposed project's test experiments on the interactions that guide the development and differentiation of the spinal cord will take advantage of some of the unique attributes of the mosquitofish. As described in detail elsewhere in this grant application, complementary work will be performed in the two laboratories.

拟议的Drs项目。波多黎各Rico-Rio-Rio Piedras（UPR-RP）和加利福尼亚理工学院（CALTECH）的Scott E. Fraser（CALTECH）的Eduardo Rosa-Molinar（UPR-RP）和Scott E. Fraser将对指导脊柱发展的细胞相互作用有更深入的了解绳索。特别是，实验将建立包含酶合成和降解视黄酸（RA）的细胞的表型，并阐明它们在引导胚胎脊髓膜状体中生发细胞的生成，迁移和命运方面的作用，特别是，次级运动神经元。实验计划基于先前的国家科学基金会在罗莎·摩林实验室（Rosa-Molinar Laboratory）进行的工作。这项工作确立了RA代谢和发育中和成熟的西部蚊子gambusia affinis finfinis affinis的脊髓的功能性神经解剖学（以下简称为“蚊子”）。拟议的项目对指导脊髓发展和分化的相互作用的测试实验将利用蚊子的某些独特属性。如本赠款申请中其他地方的详细描述，将在两个实验室中进行互补工作。