Retinoic acid in motor neuron specification

运动神经元规范中的视黄酸

基本信息

批准号：
7367951
负责人：
EDUARDO ROSA-MOLINAR
金额：
$ 39.49万
依托单位：
UNIVERSITY OF PUERTO RICO MED SCIENCES
依托单位国家：
美国
项目类别：
财政年份：
资助国家：
美国
起止时间：
至
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/7367951
关键词：
ALDH1A2 gene Ablation All-Trans-Retinol American Applications Grants Axon Biological Models Brain Stem California Cell physiology Cells Characteristics Cleft Palate Congenital Abnormality Congenital neurologic anomalies Coupled Detection Development Electroporation Embryo Environment Enzymes Ependyma Exhibits Foundations Freezing Funding Gene Expression Generations Goals Green Fluorescent Proteins Image Imagery In Situ Hybridization Individual Infant Institutes Integral Membrane Protein Kinetics Knowledge Laboratories Lasers Link Localized Metabolism Molecular Motor Motor Neurons Muscle fasciculation Nervous system structure Neuroanatomy Neuroglia Neurologic Neuronal Migration Disorder Oligonucleotides Pattern Phenotype Piedra Principal Investigator Process Protoplasmic Astrocyte Puerto Rico RNA Range Research Resource Sharing Retinal Role Rosa Scheme Science Signal Transduction Specific qualifier value Spinal Cord Spinal cord injury Staging Techniques Technology Testing Time Tretinoin Universities Vitamin A Work Zebrafish aldehyde dehydrogenase 1A2 blastomere structure cell fixing cellular targeting in vivo innovation insight malformation migration nervous system development neurodevelopment photoactivation programs research study tool

项目摘要

The proposed project of Drs. Eduardo Rosa-Molinar, the University of Puerto Rico-Rio Piedras (UPR-RP), and Scott E. Fraser of the California Institute of Technology (CalTech) will result in a greater understanding of the cellular interactions that guide the development of the spinal cord. In particular, the experiments will establish the phenotypes of the cells that contain the enzymes synthesizing and degrading retinoic acid (RA) and elucidate their role in guiding the genesis, migration, and fate of germinal cells in the ependyma of the embryonic spinal cord, specifically, secondary motor neurons. The experimental plan builds upon previous National Science Foundation funded work performed in the Rosa-Molinar laboratory. That work established both the RA metabolism and the functional neuroanatomy of the spinal cord in the developing and mature Western Mosquitofish, Gambusia affinis affinis (hereafter "mosquitofish"). The proposed project's test experiments on the interactions that guide the development and differentiation of the spinal cord will take advantage of some of the unique attributes of the mosquitofish. As described in detail elsewhere in this grant application, complementary work will be performed in the two laboratories.

拟议的Drs项目。波多黎各Rico-Rio-Rio Piedras（UPR-RP）和加利福尼亚理工学院（CALTECH）的Scott E. Fraser（CALTECH）的Eduardo Rosa-Molinar（UPR-RP）和Scott E. Fraser将对指导脊柱发展的细胞相互作用有更深入的了解绳索。特别是，实验将建立包含酶合成和降解视黄酸（RA）的细胞的表型，并阐明它们在引导胚胎脊髓膜状体中生发细胞的生成，迁移和命运方面的作用，特别是，次级运动神经元。实验计划基于先前的国家科学基金会在罗莎·摩林实验室（Rosa-Molinar Laboratory）进行的工作。这项工作确立了RA代谢和发育中和成熟的西部蚊子gambusia affinis finfinis affinis的脊髓的功能性神经解剖学（以下简称为“蚊子”）。拟议的项目对指导脊髓发展和分化的相互作用的测试实验将利用蚊子的某些独特属性。如本赠款申请中其他地方的详细描述，将在两个实验室中进行互补工作。