Using Block Copolymers to Control Cell Behavior

使用嵌段共聚物控制细胞行为

• 批准号: 6868462
• 负责人: SCOTT R SCHRICKER
• 金额: $ 12.88万
• 依托单位: OHIO STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-06-01 至 2010-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6868462
• 关键词: 3T3 cells; bioengineering /biomedical engineering; biomaterial compatibility; biomaterial development /preparation; cell adhesion; cell cell interaction; cell differentiation; cell growth regulation; cell line; cell morphology; cell proliferation; conformation; copolymer; fibronectins; osteoblasts; tissue engineering; tissue support frame

DESCRIPTION (provided by applicant): The goal of this proposal is to train the principle investigator to become an independent scientist in the field of tissue engineering. The first two years of the proposal will involve didactic training in the area of cell biology and the biological sciences. The next three years will involve executing a mentor guided research project. This project involves studying controlling the cellular response of a pre-osteoblast cell line using block copolymers. Using living or controlled free radical polymerization well-defined block copolymers from the acrylate and methacrylate families of monomer can be readily synthesized. These monomers are known to yield polymers that are biocompatible and used widely in biomaterials. The resulting morphologies from the phase segregated block copolymers can be controlled by the block size and spatial arrangement. It is expected that these morphologies can control the behavior of the cell lines. In most polymer systems, either the morphology or the surface chemistry is varied to control cell response. In the proposed system, both the morphology and surface chemistry will be varied in a controlled fashion and the effect of each on a given cellular response can be determined. This will lead to a better understanding of cell-material interaction and to the design of improved tissue engineering scaffolds. Fibronectin assays will be employed to measure the adhesion and conformation on the polymer surface. Proteins adsorbed to a polymer surface play a large role in regulating the behavior and phenotype of the cell. The adhesion and conformation data is expected to correlate to the cell responses of a MC3T3-E1cell line. Several cell responses will be measured as part of this proposal, cell proliferation and activity, cell morphology and adhesion and cell differentiation. Understanding the interaction of a systematically modulated polymer surface with a cell line will lead to the development of improved scaffold materials.

描述（由申请人提供）：本提案的目标是培训主要研究者成为组织工程领域的独立科学家。该提案的前两年将涉及细胞生物学和生物科学领域的教学培训。未来三年将涉及执行导师指导的研究项目。该项目涉及研究使用嵌段共聚物控制前成骨细胞系的细胞反应。使用活性或受控自由基聚合，可以容易地合成来自丙烯酸酯和甲基丙烯酸酯家族单体的明确嵌段共聚物。已知这些单体可产生具有生物相容性并广泛用于生物材料的聚合物。由相分离的嵌段共聚物得到的形态可以通过嵌段尺寸和空间排列来控制。预计这些形态可以控制细胞系的行为。在大多数聚合物系统中，通过改变形态或表面化学来控制细胞反应。在所提出的系统中，形态和表面化学都将以受控方式变化，并且可以确定每种对给定细胞反应的影响。这将有助于更好地理解细胞与材料的相互作用，并设计改进的组织工程支架。将采用纤连蛋白测定来测量聚合物表面上的粘附和构象。吸附到聚合物表面的蛋白质在调节细胞的行为和表型方面发挥着重要作用。粘附和构象数据预计与 MC3T3-E1 细胞系的细胞反应相关。作为该提案的一部分，将测量几种细胞反应：细胞增殖和活性、细胞形态和粘附以及细胞分化。了解系统调节的聚合物表面与细胞系的相互作用将有助于开发改进的支架材料。