Core--ANIMAL AND CELL MODEL

核心--动物和细胞模型

• 批准号: 7005298
• 负责人: James J Manfredi
• 金额: $ 8.75万
• 依托单位: ICAHN SCHOOL OF MEDICINE AT MOUNT SINAI
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-07-01 至 2010-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7005298
• 关键词: animal breeding; animal colony; biomedical facility; cell line; cellular oncology; genetic models; genetic regulation; genetically modified animals; histopathology; laboratory mouse; neoplasm /cancer genetics; p53 gene /protein; serial analysis of gene expression; tissue /cell preparation

Insights gained primarily from in vitro models of p53 regulators and effectors within our research program have led to a critical need for in vivo validation and the ability to gain further understanding using in vivo models. The phenotypes that are actively being examined in the p53 paradigm are no longer simply a matter of cell death, growth arrest or senescence. Instead, the role of p53 activation in vivo will likely have different consequences in different cell and tissue types and will likely differ with regard to developmental stages. This Core maintains stocks of human and animal tumor cell lines as well as genetically engineered cell lines that are used within the Program. The Core also derives and maintains adequate stocks of early passage aliquots of frozen primary cells derived from mouse strains for distribution to the investigators in the Program. The research efforts of our productive and collaborative program greatly benefit from an in vivo models component, which maintains small breeding colonies of critically needed mouse strains for genetic and cell biologic investigations by our investigators. The high cost of animal studies in barrier facilities can be reduced substantially by the availability of shared technical support for mouse breeding and genetic analyses and for shared immuno-histochemistry. The cost effectiveness is further emphasized by the shared use of the same genetically modified strains by several investigators and projects within the Program. The Cell and Animal Model Core will derive and maintain human and mouse cell lines as well as adequate stocks of early passage aliquots of frozen primary cells utilized by the projects. The Core will also acquire and maintain steadily used mouse models (transgenic, knockouts, etc.) and primary cells derived from these mice for distribution to the investigators in the Program. Lastly, the core will provide expertise and guidance in the preparation of tissue and/or embryos for analyses of gene expression (protein and mRNA in situ) and histopathology.

我们的研究项目中主要从 p53 调节子和效应子的体外模型获得的见解导致迫切需要体内验证以及使用体内模型获得进一步了解的能力。 p53范式中正在积极研究的表型不再仅仅是细胞死亡、生长停滞或衰老的问题。相反，体内 p53 激活的作用可能在不同的细胞和组织类型中产生不同的后果，并且可能在发育阶段方面有所不同。该核心保留了该计划中使用的人类和动物肿瘤细胞系以及基因工程细胞系的库存。核心还获得并维持足够的来自小鼠品系的冷冻原代细胞的早期传代等分试样的库存，以分发给该计划的研究人员。我们富有成效的合作项目的研究工作极大地受益于体内模型组件，该组件维持了我们的研究人员进行遗传和细胞生物学研究急需的小鼠品系的小型繁殖群体。通过为小鼠育种和遗传分析以及共享免疫组织化学提供共享技术支持，可以大大降低屏障设施中动物研究的高成本。共享使用进一步强调了成本效益 该计划内的几位研究人员和项目对相同的转基因菌株进行了研究。 细胞和动物模型核心将衍生和维持人类和小鼠细胞系以及 项目使用的冷冻原代细胞的早期传代等分试样的充足库存。核心还将获取并维护稳定使用的小鼠模型（转基因、基因敲除等）以及源自这些小鼠的原代细胞，以分发给该计划的研究人员。最后，该核心将为基因表达（原位蛋白质和 mRNA）和组织病理学分析的组织和/或胚胎的制备提供专业知识和指导。