Global analysis of gene regulation in the midline CNS

中枢神经系统基因调控的整体分析

• 批准号: 7076869
• 负责人: JOSEPH B KEARNEY
• 金额: $ 5.04万
• 依托单位: UNIV OF NORTH CAROLINA CHAPEL HILL
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-07-23 至 2007-07-22
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7076869
• 关键词: Drosophilidae; RNA interference; arthropod genetics; central nervous system; confocal scanning microscopy; developmental genetics; fluorescent in situ hybridization; functional /structural genomics; genetic mapping; genetic regulation; genetic screening; glia; high throughput technology; microarray technology; mutant; postdoctoral investigator; protein localization

DESCRIPTION (provided by applicant): Specialized midline precursors of the developing Drosophila embryo give rise to specific sets of neurons and gila, and serve as an excellent model system for the study of CNS development and function. Using available data, our lab has constructed a database of 297 putative midline-expressed genes. Since the exact expression patterns of a majority of these genes are unknown, a major goal of this proposal is to use high throughput RNA in situ hybridization to map the expression patterns of these genes during CNS midline development. This analysis will provide (1) a refined molecular map of the midline CNS and (2) serve as a reference for the analysis of midline CNS gene transcription. To determine which of these genes are involved in glial development, midline-glial-expressed genes will be functionally screened for glial phenotypes using RNA interference and available genetic mutants. For glial genes encoding transciption factors, in situ expression pattern screening and microarray analysis will be used to identify transcriptional changes in mutant genetic backgrounds. This analysis will provide a clearer understanding midline CNS development and the transcriptional pathways that regulate midline glial development and function.

描述（由申请人提供）：发育中的果蝇胚胎的专门中线前体可引起特定的神经元和吉拉集合，并作为研究中枢神经系统开发和功能的出色模型系统。使用可用的数据，我们的实验室构建了一个数据库，该数据库由297个假定的中线表达基因构建。由于这些基因大多数的确切表达模式尚不清楚，因此该建议的主要目标是使用高吞吐量RNA原位杂交来绘制中枢神经系统中线开发过程中这些基因的表达模式。该分析将提供（1）中线CNS的精制分子图，（2）作为中线CNS基因转录分析的参考。为了确定哪些基因参与神经胶质发育，将使用RNA干扰和可用的遗传突变体在功能上筛选中线表达的基因。对于编码转递因子的神经基因，原位表达模式筛选和微阵列分析将用于识别突变遗传背景中的转录变化。该分析将提供更清楚的中线开发以及调节中线幻觉发展和功能的转录途径。