BD FACSAria Flow Cytometer Cell Sorter

BD FACSAria 流式细胞仪细胞分选仪

• 批准号: 6877610
• 负责人: CHOU-ZEN GIAM
• 金额: $ 37.76万
• 依托单位: HENRY M. JACKSON FDN FOR THE ADV MIL/MED
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-02-15 至 2006-02-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6877610
• 关键词: biomedical equipment purchase; cell biology; cytology; flow cytometry

DESCRIPTION (provided by applicant): This Shared Instrumentation Grant proposal is for the purchase of the BD FACSAria Flow Cytometer Cell Sorter. This is a state-of-the-art fully digital, three laser (488nm, 633nm and 407nm) Flow Cytometer Cell Sorter. It is capable of sorting two to four populations at an acquisition rate of up to 70,000 cells per second. This request includes several options. The water recirculator will provide optimal temperature control for the sample and sorted fractions. The Automated Cell Deposition Unit (ACDU) option is an integrated cell deposition system for multi-well plate and slide sorting. The aerosol management system option will enable sorting of biologically hazardous samples. The HP XW4000 Workstation provides a powerful data management system. The new BD FACSAria cell sorter will be housed at USUHS in the Biomedical Instrumentation Center (BIC), Flow Cytometry Facility, Room G235, and will be operated and maintained by the Flow Cytometry Facility Director, Karen M. Wolcott. The Principal Investigator, William C. Gause, will provide administrative/scientific oversight in consultation with an internal advisory committee. The BD FACSAria is requested as a replacement for the current flow cytometer cell sorter, a 13-year old Beckman Coulter EPICS ELITE ESP. Many requests for sorting have been declined due to the inability of this antiquated instrument to support the needs of many research protocols, especially those of the NIH-supported investigators identified in this grant. First priority will be given to the NIH-supported scientists who are participating in this grant proposal. They include: 1.) Brumeanu, Teodor D. - Prevention of Type 1 Diabetes by soluble MHC II- peptides; 2.) Cutler, Mary Lou - Role of Ras Effectors in Mammary Differentiation; 3.) Darling, Thomas N. - Tumorigenesis in Tuberous Sclerosis; 4.) Gause, William C. - Regulation of Th2 cell commitment, differentiation, and cell cycle progression in vivo; 5.) Giam, Chou-Zen - Molecular Biology and Pathogenesis of HTLV-1; 6.) Pollard, Harvey B. - Regulation of Inflammatory Pathways in Cystic Fibrosis; 7.) Provencio, Ignacio Isolation and Characterization of Melanopsin-Containing Retinal Ganglion Cells; 8.) Schaefer, Brian C. - Antigen receptor regulation of NF-KB signaling in lymphocyte activation and tumorigenesis; 9.) Snapper, Clifford M. - Dendritic and T cells in antibacterial Ig responses; 10.) Tsokos, George C. - Regulation of lymphocyte function during Systemic Lupus Erythematosus (SLE); and 11.) Via, Charles S. - Immunopathogenesis of Lupus. It is anticipated that this group of investigators will require 95% of the total usage of the instrument. The remaining 5% will be allocated to USUHS, NIH-supported research scientists or other individuals in the USUHS community on a first come, first served basis. Sorting time may be made available to investigators outside of the USUHS community from time to time when the instrument would otherwise not be used.

描述（由申请人提供）：此共享的仪器赠款提案用于购买BD FACSARIA流式细胞仪细胞分隔器。这是一个最先进的完全数字，三个激光器（488nm，633nm和407nm）流式细胞仪分隔器。它能够以每秒高达70,000个单元的采集速率对两到四个种群进行排序。此请求包括几个选项。水回流器将为样品和分类分数提供最佳的温度控制。自动细胞沉积单元（ACDU）选项是用于多孔板和幻灯片排序的集成细胞沉积系统。气溶胶管理系统选项将使生物学上的危险样品分类。 HP XW4000工作站提供了强大的数据管理系统。新的BD FACSARIA细胞分系程序将安置在生物医学仪器中心（BIC），流式细胞仪设施，G235室的USUHS，并将由流式细胞仪设施主管Karen M. Wolcott进行操作和维护。首席调查员威廉·盖斯（William C. Gause）将在与内部咨询委员会协商时提供行政/科学监督。 BD FACSARIA被要求作为当前流式细胞仪分隔器的替代，这是13岁的Beckman Coulter Epics Elite Esp。由于这种过时的工具无法支持许多研究方案的需求，尤其是该赠款中确定的NIH支持的研究人员的需求，因此已经拒绝了许多分类请求。参加该赠款提案的NIH支持的科学家将获得首要任务。它们包括：1。）Brumeanu，Teodor D.-可溶性MHC II-肽预防1型糖尿病； 2.）Cutler，Mary Lou- RAS效应子在乳腺分化中的作用； 3.）亲爱的，托马斯·N。 - 结节硬化症中的肿瘤发生； 4.）Gause，William C.-对体内Th2细胞承诺，分化和细胞周期进展的调节； 5.）Giam，Chou-Zen- HTLV-1的分子生物学和发病机理； 6.）Pollard，Harvey B.-囊性纤维化中炎症途径的调节； 7.）Provencio，含有黑色素的视网膜神经节细胞的Ignacio分离和表征； 8.）Schaefer，Brian C.-淋巴细胞激活和肿瘤发生中NF -KB信号传导的抗原受体调节； 9.）Snapper，Clifford M.-抗菌Ig反应中的树突状和T细胞； 10.）Tsokos，George C.-全身性红斑狼疮（SLE）期间淋巴细胞功能的调节；和11.）通过Charles S.-狼疮的免疫发作。预计该研究人员将需要该工具总使用量的95％。其余的5％将分配给USUHS，由NIH支持的研究科学家或USUHS社区中的其他人首先获得。分类时间可能会不时地向USUHS社区以外的调查人员提供，否则将不使用该乐器。

项目成果

Complex cell cycle abnormalities caused by human T-lymphotropic virus type 1 Tax.

由人类 T 淋巴细胞病毒 1 型 Tax 引起的复杂细胞周期异常。

• DOI: 10.1128/jvi.00086-10
• 发表时间: 2011
• 期刊: Journal of virology
• 影响因子: 5.4
• 作者: Yang,Liangpeng;Kotomura,Naoe;Ho,Yik-Khuan;Zhi,Huijun;Bixler,Sandra;Schell,MichaelJ;Giam,Chou-Zen
• 通讯作者: Giam,Chou-Zen