TEM with Digital and Cryopreparation Systems

配备数字和冷冻制备系统的 TEM

• 批准号: 6576822
• 负责人: ANDREW P EVAN
• 金额: $ 38.66万
• 依托单位: INDIANA UNIV-PURDUE UNIV AT INDIANAPOLIS
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-06-01 至 2004-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6576822
• 关键词: biomedical equipment purchase; biomedical equipment resource; cell biology; cryoelectron microscopy; cytology; digital imaging; immunocytochemistry; transmission electron microscopy

DESCRIPTION (provided by applicant): The purpose of the application is to obtain funding to replace the existing Philips 400 TEM in the shared-user EM Center in the Department of Anatomy and Cell Biology with a state-of-the-art TEM, the JEOL 1230. The Philips 400 TEM was purchased with NSF funds in 1979 and has served over 40 different researchers over the last 23 years, but this machine is presently unreliable and outdated. Since the acquisition of the Philips 400 TEM, significant technical advances have occurred in transmission electron microscopes which include ease of use and extended capabilities like digital image recording and ultracryo-microscopy. The new TEM will be housed in a 1789 sq. ft. laboratory dedicated to electron microscopy, supervised by a full-time faculty member, Dr. Vincent Gattone and managed by a full-time, highly qualified EM specialist, Ms. Caroline Miller. The microscope will be available to all investigators within the Indiana University-Purdue University campus at Indianapolis which includes Schools of Dentistry, Medicine, Science and Engineering & Technology. However, the majority of the research projects will come from ten (10) laboratories. These investigators have been identified as "major users" of the new instrument. Dr. Robert Bacallao will employ a variety of biochemical assays for protein sorting, immunohistochemical and ultrastructural analyses of Golgi complex dysfunction following ischemic injury. Dr. Andrew Evan will use imunohistochemical and digital imaging to precisely correlate sites of renal crystal deposition with known inhibitors of stone formation. Dr. Loren Field will use TEM to correlate structural changes in cardiomyocytes after specific pathological conditions. Dr. Lincoln Ford requires digital TEM images to accurately measure changes in smooth muscle thick filaments during contractility. Dr. Susan Gunst needs digital TEM images to evaluate the cellular mechanisms that regulate the response of airway smooth muscle to mechanical forces generated during breathing. Dr. James McAteer's studies require the high resolution of the TEM to detect subtle damage to the vascular endothelium induced by shock wave lithotripsy. Dr. Bruce Molitoris will employ immunohistochemistry to determine the cellular, biochemical and molecular mechanisms responsible for ischemia induced membrane changes. Dr. Carrie Phillips will use immunogold EM to evaluate the cellular localization of inversin protein in the inv mutant mouse model of PKD. Dr. Zao Xu will use TEM to investigate the nature of cell death, e.g. necrosis or apoptosis, in neostriatum of the rat after transient global schemia. Dr. Richard Gregory will employ immunohistochemistry to examine the role of a Streptococcus mutans 65 kDa fimbrial protein binding as a mechanism for the induction of dental carries.

描述（由申请人提供）： 该申请的目的是获得资金，以使用最先进的TEM，JEOL 1230来代替解剖和细胞生物学系的共享用户EM中心现有的TEM。自从获得飞利浦400 TEM的获取以来，在传输电子显微镜中发生了重大的技术进步，包括易用性和扩展功能（例如数字图像记录和超丙酰基微镜）。新的TEM将安置在1789平方英尺的实验室中，该实验室专门针对电子显微镜，该实验室由全职教职员工Vincent Gattone博士监督，并由一位全职，高素质的EM专家Caroline Miller女士管理。显微镜将向印第安纳大学普渡大学校园内的所有研究人员使用，其中包括牙科，医学，科学与工程与技术。但是，大多数研究项目将来自十（10）个实验室。这些调查人员已被确定为新工具的“主要用户”。罗伯特·巴卡洛（Robert Bacallao）博士将采用各种生化测定，用于缺血性损伤后高尔基体复合功能障碍的蛋白质分类，免疫组织化学和超微结构分析。安德鲁·埃文（Andrew Evan）博士将使用毫米组织化学和数字成像来精确地将肾脏晶体沉积的位点与已知的石材形成抑制剂相关联。洛伦·菲尔德（Loren Field）博士将使用TEM将特定病理条件后心肌细胞的结构变化相关联。林肯·福特（Lincoln Ford）博士需要数字TEM图像，以准确测量收缩性过程中平滑肌厚细丝的变化。 Susan Gunst博士需要数字TEM图像来评估调节气道平滑肌对呼吸过程中机械力的响应的细胞机制。詹姆斯·麦卡特（James McAteer）博士的研究要求TEM的高分辨率高分辨率，以检测冲击波写碎石诱导的血管内皮的细微损害。 Bruce Molitoris博士将采用免疫组织化学来确定负责缺血诱导膜变化的细胞，生化和分子机制。 Carrie Phillips博士将使用免疫质量EM来评估PKD的Inv突变小鼠模型中因弗氏蛋白蛋白的细胞定位。 Zao Xu博士将使用TEM研究细胞死亡的性质，例如瞬时全球模态后大鼠新纹状体中的坏死或凋亡。理查德·格雷戈里（Richard Gregory）博士将采用免疫组织化学来研究65 kDa纤维蛋白结合的链球菌突变的作用，作为诱导牙齿携带的机制。