Acquisition of the Agilent Cytation C10 confocal imaging reader for enhancing biomedical research excellence at Clemson University

采购 Agilent Cytation C10 共焦成像阅读器，以提高克莱姆森大学的生物医学研究卓越性

• 批准号: 10798537
• 负责人: JAMES Culvin MORRIS
• 金额: $ 29.61万
• 依托单位: CLEMSON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-07-15 至 2027-05-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10798537
• 关键词: Advisory Committees; African Trypanosomiasis; Amebic colitis; Antifungal Agents; Antiparasitic Agents; Area; Aspergillosis; Aspergillus fumigatus; Assistantship; Award; Azoles; Bioinformatics; Biology; Biomedical Research; Bioterrorism; C10; Cardiovascular Diseases; Cell Separation; Centers of Research Excellence; Chagas Disease; Classification; Collaborations; Colon Carcinoma; Communicable Diseases; Communication; Complex; Country; Cryptococcus; Detection; Development; Diagnosis; Disease; Dynein ATPase; Dysentery; Educational workshop; Environment; Environmental Risk Factor; Equipment; Evolution; Faculty; Faculty Recruitment; Fostering; Funding; Fungal Meningitis; Genomics; Global Change; Goals; Grant; Helminths; Host Defense; Human; Image; Incidence; Infection; Infrastructure; Institution; Interdisciplinary Study; Knowledge; Leadership; Light; Macrophage; Malaria; Medical; Meningitis; Mentors; Modeling; Musculoskeletal Diseases; Oxidants; Parasites; Parasitic Diseases; Phase; Pilot Projects; Postdoctoral Fellow; Poverty; Probability; Program Research Project Grants; Reader; Regulation; Research; Research Personnel; Resource Allocation; Role; Scientist; South Carolina; Stress; Stroke; Student recruitment; Toxoplasmosis; Training; Trichomonas Infections; Trypanosoma; United States National Institutes of Health; Universities; Vaccines; Virulence; Zebrafish; base; climate change; college; confocal imaging; cytate; dietary supplements; drug discovery; emerging pathogen; graduate student; human disease; imaging facilities; innovation; insight; lipidomics; member; multidisciplinary; neglect; neglected tropical diseases; pathogen; primary amebic meningoencephalitis; programs; recruit; response; skills; success; targeted treatment; tenure track; tissue regeneration; transmission process; vector

PROJECT SUMMARY/ABSTRACT Eukaryotic pathogens are the causative agents of some of the most devastating and intractable diseases of humans, including malaria, amebic dysentery, African sleeping sickness, Chagas disease, trichomoniasis, aspergillosis, cryptococcal fungal meningitis, toxoplasmosis and primary amebic meningoencephalitis (PAM). The global impact of these diseases is immense. It is noteworthy that many of these pathogens are the causative agents of neglected tropical diseases (NTDs), neglected diseases of poverty (NDPs) and/or are classified as bioterrorism agents. Importantly, infections caused by eukaryotic pathogens are increasing in the US due to globalization. The primary goal of this COBRE proposal is to increase the number of NIH-funded scientists in the state of South Carolina by supporting a world-class research center, the Eukaryotic Pathogens Innovation Center (EPIC), at Clemson University (CU). The scientific focus of EPIC is a multidisciplinary study of important global eukaryotic pathogens. EPIC is the first-and-only in the state with a focus on infectious diseases, and the first- and-only in the country with a focus on eukaryotic pathogens. Four projects from target junior investigators will be supported. Their projects are 1) Regulation mechanisms of Trypanosome brucei axonemal dynein, 2) Functional roles of ncRNA, afu-182, in azole response and pathobiology of Aspergillus fumigatus, 3) Investigating the function of macrophages in the efficacy of anti-fungal drugs in larval zebrafish, and 4) Evaluating anti-parasitic diazacyclobutenes. These investigators will be matched with external and internal mentors who are established NIH-funded researchers. The projects will be supported by a well-organized Administrative Core and two state-of-the-art Scientific Cores in Genomics, and Imaging/Cell Sorting. The center also has a substantial infrastructure base and significant institutional support. For example, CU will recruit two additional faculty members and two post-doctoral fellows to expand activities of this center over the course of the project period. Pledges of graduate assistantships, equipment, and space further exemplify the institutional commitment. The continuation of this COBRE-funded center will significantly expand research in South Carolina and will facilitate recruitment, training, and retention of a critical mass of investigators with cross-disciplinary skills in this important research area.

项目摘要/摘要 真核生物病原体是一些最具破坏性和棘手的疾病的病原体 人类，包括疟疾，阿米比克痢疾，非洲昏睡病，chagas病，毛诺病， 曲霉病，隐球菌真菌性脑膜炎，弓形虫病和原发性肌膜脑膜脑炎（PAM）。 这些疾病的全球影响是巨大的。值得注意的是，其中许多病原体都是因果关系 被忽视的热带疾病（NTD）的药物，被忽视的贫困疾病（NDP）和/或被归类为 生物恐怖剂。重要的是，由于真核病原体引起的感染在美国增加 全球化。该索布尔提案的主要目标是增加NIH资助的科学家的数量 南卡罗来纳州通过支持世界一流的研究中心，真核病原体创新中心 （史诗），克莱姆森大学（CU）。史诗的科学重点是重要全球的多学科研究 真核病原体。史诗是该州的第一和只专注于传染病，第一个 仅在该国关注真核病原体。目标初级调查人员的四个项目将 得到支持。他们的项目是1）Brucei Brucei Axonemal Dynein的调节机制，2） NCRNA，AFU-182的功能作用，在曲霉烟曲霉的唑反应和病理学中，3） 研究巨噬细胞在幼虫斑马鱼中抗真菌药物功效中的功能，4）评估 抗寄生虫氯氮酰丁烯。这些调查人员将与外部和内部导师匹配 已建立的NIH资助的研究人员。这些项目将得到组织良好的行政核心的支持， 基因组学和成像/细胞分选的两个最先进的科学核心。该中心也有很大的 基础设施基础和大量机构支持。例如，CU将招募另外两个教师 成员和两个博士后研究员在项目期间扩大该中心的活动。 研究生助理，设备和空间的承诺进一步体现了机构的承诺。这 这个由毛茸茸资助的中心的延续将大大扩大南卡罗来纳州的研究，并将促进 在这个重要的 研究领域。

项目成果

Impact of fetal exposure to mycotoxins on longissimus muscle fiber hypertrophy and miRNA profile.

• DOI: 10.1186/s12864-022-08794-0
• 发表时间: 2022-08-16
• 期刊: BMC GENOMICS
• 影响因子: 4.4
• 作者: Greene, M. A.;Udoka, A. N. S.;Powell, R. R.;Noorai, R. E.;Bruce, T.;Duckett, S. K.
• 通讯作者: Duckett, S. K.

Draft Genome Sequence of Exophiala Strain HKRS030, a Fungus Capable of Reducing Iron but Not Nitrate.

• DOI: 10.1128/mra.00615-22
• 发表时间: 2023-03-16
• 期刊: Microbiology resource announcements
• 影响因子: 0.8

Genetic Diversity and Fungicide Sensitivity of Cytospora plurivora on Peach.

桃多食胞孢菌的遗传多样性和杀菌剂敏感性。

• DOI: 10.1094/pdis-04-22-0790-re
• 发表时间: 2023
• 期刊: Plant disease
• 影响因子: 4.5
• 作者: Baker,StephenT;Froelich,MarthaH;Boatwright,Harriet;Wang,Hehe;Schnabel,Guido;Kerrigan,Julia
• 通讯作者: Kerrigan,Julia

Radiopaque Iodosilane-Coated Lipid Hybrid Nanoparticle Contrast Agent for Dual-Modality Ultrasound and X-ray Bioimaging.

• DOI: 10.1021/acsami.2c09104
• 发表时间: 2022-11
• 期刊: ACS applied materials & interfaces
• 影响因子: 9.5
• 作者: M. Attia;R. Akasov;Nancy M. Elbaz;T. Owens;Evan C Curtis;Soham Panda;R. Santos-Oliveira;F. Alexis;Forrest M. Kievit;Daniel. C. Whitehead

Comparison of cell viability assessment and visualization of Aspergillus niger biofilm with two fluorescent probe staining methods.

• DOI: 10.1016/j.bioflm.2022.100090
• 发表时间: 2022-12
• 期刊: Biofilm
• 影响因子: 6.8