Instrumentation for Confocal Imaging and Analysis

共焦成像和分析仪器

• 批准号: 6440979
• 负责人: BRUCE A STANLEY
• 金额: $ 34.01万
• 依托单位: PENNSYLVANIA STATE UNIV HERSHEY MED CTR
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-07-01 至 2003-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6440979
• 关键词: biomedical equipment purchase; cell biology; confocal scanning microscopy; cytology

High-resolution visualization of cellular compartments and components is crucial to a wide variety of research projects at the Penn State College of Medicine, including co-localization studies, studies of macromolecular interactions using FRET analysis, and transport/kinetic studies of movement within cells. This proposal requests funds to replace our aged and no longer adequate Zeiss 210 upright confocal microscope with a new Leica TCS SP2 inverted microscope confocal system. The upgrading of our confocal microscopy instrumentation will enable us to add a crucial service in our shared Core Facilities which will be used by numerous NIH-funded investigators, twelve of whom are still included as the major users group in this proposal. Although the current confocal microscope is still adequate for very simple studies, and has produced images used in numerous publications from our researchers, the lack of a third laser line, the pack of 3D reconstruction software, the inability to keep images in registration when changing dichroic filters or changing to transmission optics, the cumbersome interface, and the poor service record for the 210 make it impossible to use for most current applications and research needs. For these reasons, our researchers have either had to go elsewhere for their confocal needs (1 100 mile drive to our State College campus), or find alternative, less-than-optimal solutions to addressing their research aims. Since the current confocal is known to be very difficult to use and often not functioning, this has also prevented new researchers, with new projects that would benefit from the use of a good confocal system, from establishing investigative systems using confocal microscopy. In addition to our own testing and extensive consultation with imaging scientists with imaging scientists concerning competing confocal systems (BioRad, Olympus, Nikkon, Zeiss and Leica), we have spoken at length with imaging experts at six different academic locations, and also investigated the possibility of upgrading our current system (Microcosm, Columbia, MD). The requested Leica TCS SP2 confocal system is a state-of-the-art system that will meet all of our current and foreseeable needs for confocal microscopy, and its tuneable emission detection allows both optimal separation of current fluors and the flexibility to adapt new fluorescent problems as they are developed. The requested instrument will provide crucial support to our major users group's projects, comprising 21 NIH grants totally over $2 million in current yearly direct costs, as well as aiding the development of new research projects in our larger research community.

细胞室和成分的高分辨率可视化对于宾夕法尼亚州立医学院的各种研究项目至关重要，包括共同定位研究，使用FRET分析对大分子相互作用的研究以及细胞内运动的运输/动力学研究。该提案要求资金用新的Leica TCS SP2倒置显微镜共聚焦系统代替我们的老化和不足的Zeiss 210直立共聚焦显微镜。 升级我们的共聚焦显微镜仪器将使我们能够在共享的核心设施中添加至关重要的服务，该设施将被众多由NIH资助的调查人员使用，其中十二名仍被包括在本提案中作为主要用户组。尽管目前的共聚焦显微镜仍然足够简单的研究，并且已经制作了我们研究人员的众多出版物中使用的图像，但缺乏第三个激光系列，一包3D重建软件包，无法在更改二氯滤波器时保持图像在更改Dichroic Filters或更改为cumbersome Optics，cumbersome界面，以及对210的不良服务中的不良服务时，无法使用210的服务。由于这些原因，我们的研究人员要么不得不出于共同需求（前往我们州立大学校园100英里的车程），要么不得不去其他地方，要么找到替代的，不太理想的解决方案来满足其研究目标。由于已知当前的共聚焦很难使用，而且通常不正常，因此这也阻止了新的研究人员，新项目将受益于使用良好共聚焦系统的新项目，无法使用共聚焦显微镜建立调查系统。除了与成像科学家与成像科学家有关竞争共焦系统（Biorad，Olympus，Olympus，Nikkon，Zeiss和Leica）的测试和广泛的咨询外，我们还与六个不同学术地点的成像专家进行了详尽的交谈，还调查了我们当前系统（Microcosm，Columbia，Columbia，M.Md）的可能性。请求的Leica TCS SP2共聚焦系统是一种最先进的系统，它将满足我们对共聚焦显微镜的所有当前和可预见的需求，并且其可调的发射检测既允许当电流荧光的最佳分离，又可以随着新的荧光问题而适应新的荧光问题。所请求的工具将为我们的主要用户小组的项目提供至关重要的支持，其中包括21个NIH赠款超过200万美元的年度直接成本，并有助于我们在我们较大的研究社区中开发新的研究项目。