Laser Scanning Confocal Microscope

激光扫描共焦显微镜

• 批准号: 6877450
• 负责人: RALPH A. NIXON
• 金额: $ 43.69万
• 依托单位: NATHAN S. KLINE INSTITUTE FOR PSYCH RES
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-09-01 至 2006-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6877450
• 关键词: bioimaging /biomedical imaging; biomedical equipment purchase; cell biology; confocal scanning microscopy; molecular /cellular imaging

DESCRIPTION (provided by applicant): Funds are requested for the purchase of a Zeiss LSM510 laser confocal microscope. There is a strong justification for a new confocal laser microscope to replace our existing largely outdated model that currently serves all of the NIH-funded researchers at our Institute. The new microscope will extend the capabilities of our imaging facility, while providing a much-needed and valuable research tool for the seven applicant investigators and their co-investigators, which form the major user group. Instrumentation comparable to that requested is not available near NKI. The analysis and interpretation of structural and cell biological data by the best technological means available, including laser confocal microscopy, is of critical importance to the continuation and enhancement of ongoing research programs, and to the evolution of these programs in new directions. Acquisition of the new instrument will enable scientists throughout our institute to explore a diversity of topics organized around the theme of basic and translational neuroscience of neurodegenerative and neuropsychiatric diseases. All of the NIH-funded programs referenced in the application have components that require and would be strengthened by confocal laser microscopy. These are summarized as follows: 1) AD-related proteins following therapeutic intervention in animal models; 2) Expression Profiling in Neurodegenerative Disorders; 3) Cystatin C in cerebral hemorrhage and in Alzheimer's disease (AD); 4) The endocytic pathway in AD and Down's Syndrome; 5) The endosomal-autophagic-lysosomal system in AD Pathogenesis; 6) The role of disrupted cholesterol metabolism in AD pathogenesis; and 7) Signal Transduction in Neuropsychiatric Disease. An enhancement of the Institute's imaging capabilities will allow all applicant investigators to pursue funded research programs with greater efficiency and depth, to expand existing programs in new directions and to develop new programs.

描述（由申请人提供）：要求购买Zeiss LSM510激光共聚焦显微镜的资金。对于新的共聚焦激光显微镜，有很大的理由来替换我们现有的过时的模型，该模型目前为我们研究所的NIH资助的所有研究人员提供服务。新的显微镜将扩展我们的成像设施的功能，同时为构成主要用户组的七个申请人研究人员及其共同研究人员提供急需和宝贵的研究工具。 NKI附近没有与所要求的仪器相当。通过包括激光共聚焦显微镜在内的最佳技术手段对结构和细胞生物学数据的分析和解释对于正在进行的研究计划的延续和增强以及这些程序在新方向上的演变至关重要。收购新工具将使整个研究所的科学家能够探索围绕神经退行性和神经精神疾病的基本和转化神经科学主题组织的多样性。应用程序中引用的所有NIH资金资助的程序的组成部分都需要通过共聚焦激光显微镜来加强并且将加强。这些总结如下：1）在动物模型中治疗干预后与AD相关的蛋白质； 2）神经退行性疾病中的表达分析； 3）在脑出血和阿尔茨海默氏病（AD）中的胱抑素C； 4）AD和Dows综合征的内吞途径； 5）AD发病机理中的内体 - 自噬 - 淋巴液系统； 6）破坏胆固醇代谢在AD发病机理中的作用； 7）神经精神疾病的信号转导。 对研究所的成像功能的增强将使所有申请人调查人员能够以更高的效率和深度来追求资助的研究计划，从而在新方向扩展现有计划并开发新计划。