Radiance 2100 AG-2Q Confocal Multiphoton System

Radiance 2100 AG-2Q 共焦多光子系统

基本信息

批准号：
6877455
负责人：
ZHENG GANG ZHANG
金额：
$ 50万
依托单位：
HENRY FORD HEALTH SYSTEM
依托单位国家：
美国
项目类别：
财政年份：
2005
资助国家：
美国
起止时间：
2005-04-01 至 2006-03-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/6877455
关键词：
angiogenesis biomedical equipment purchase blood brain barrier cell biology cellular pathology confocal scanning microscopy molecular /cellular imaging nervous system disorder neural plasticity stroke vascular endothelial growth factors

项目摘要

DESCRIPTION (provided by applicant): With increasing numbers of NIH funded investigators using animal models to study molecular mechanisms and treatment of diseases, there is a compelling need to image tissue structure and function in situ, in vivo, over time without damaging tissue. The major system-related limitation of LSCM imaging of living tissues is photodamage caused by the intense laser illumination and limited depth penetration of the laser beam. Two photon laser scanning microscope (TPLSM) technology overcomes these limitations. TPLSM is fast becoming the imaging method of choice to study fundamental biological processes in living animals. Our preliminary data demonstrated that the two photon microscopy can be successfully used on the living mouse after stroke. We request funds to purchase a Radiance 2100 AG-2Q Confocal Multiphoton System to be employed in an array of NIH funded grants, including 3 P0ls. The new system will be utilized for studies of molecular and pathophysiological mechanisms underlying thrombosis, blood brain barrier disruption, angiogenesis, axonal and dendritic plasticity, brain tumor, chondrocyte apoptosis, and hypertension. Imaging changes in immunofluorescent signals and green fluorescent protein (GFP) signals are a critical part of each of these N IH funded grants. The use of Radiance 2100 AG-2Q Confocal Multiphoton System will allow investigators to greatly expand their proposed experiments to image changes in tissue structure and function in situ over time without damaging or sectioning tissue. This will greatly augment to accomplishments of these funded grants and research at Henry Ford Health Science Center as well as provide a major resource to the research community in Metropolitan Detroit. Furthermore, data obtained from the Radiance 2100 AG-2Q Confocal Multiphoton System will provide new insights into mechanisms underlying our proposed studies, permitting us to generate new hypotheses to be tested in grant renewal and new grant applications.

描述（由申请人提供）：使用动物模型来研究分子机制和疾病治疗的NIH资助的研究人员的数量越来越多，迫切需要对组织结构和现场，体内，随着时间的流逝而不会损害组织。活组织LSCM成像的主要系统相关的局限性是激光照明引起的光损伤和激光束的深度渗透有限。两种光子激光扫描显微镜（TPLSM）技术克服了这些局限性。 TPLSM迅速成为研究活动物基本生物学过程的首选成像方法。我们的初步数据表明，两种光子显微镜可以在中风后成功地用于活小鼠。我们要求资金购买Radiance 2100 AG-2Q共聚焦多光系统，该系统用于一系列NIH资助的赠款，包括3个P0L。新系统将用于研究血栓形成，血脑屏障破坏，血管生成，轴突和树突状可塑性，脑瘤，软骨细胞凋亡和高血压的分子和病理生理机制。免疫荧光信号和绿色荧光蛋白（GFP）信号的成像变化是这些N IH资助的每一种的关键部分。辐射2100 AG-2Q共聚焦多光子系统的使用将使研究人员能够大大扩展其提出的实验，以图像随着时间的推移而无需损害或切除组织的情况，就可以在本地进行组织结构和功能。这将大大增加亨利·福特健康科学中心这些资助的赠款和研究的成就，并为底特律大都会的研究社区提供了主要资源。此外，从Radiance 2100 AG-2Q共聚焦多光子系统获得的数据将提供有关我们提出的研究基础的机制的新见解，使我们能够在赠款续订和新的赠款应用中产生新的假设。