FLOW CYTOMETRY FACILITY HIGH SPEED SORTER

流式细胞术设备高速分选机

• 批准号: 6877619
• 负责人: BICE PERUSSIA
• 金额: $ 25.67万
• 依托单位: THOMAS JEFFERSON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-02-15 至 2006-02-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6877619
• 关键词: biomedical equipment purchase; cell biology; cytology; flow cytometry

DESCRIPTION (provided by applicant): This proposal is to acquire a state-of-the-art flow cytometer with sorting capabilities in order to upgrade the multi-user Flow Cytometry Facility of the Kimmel Cancer Center, Thomas Jefferson University, and thus optimally support the research activities of the NIH-funded members of the Cancer Center. We request to purchase a Cytomation MoFlo(TM) HTS Ultra High-Speed Sort Flow Cytometer with capability of high speed 4-way sorting, of routine simultaneous analysis of greater than 4 fluorochromes, and with the added capability of single cell deposition. Currently, the KCC Flow Cytometry Facility is the sole facility of this type on the TJU campus. It is equipped with a 12-year old Coulter EPICS Elite ESP, which is also a sorting instrument, and two analyzers: a 4-year old Coulter EPICS XL-MCL, and a 15- year old Becton-Dickinson FACSCalibur Benchtop Analyzer. A newly acquired BD Aria Sorter is physically present (although yet to be installed in a newly to-be-developed facility) in the Neurology Department at TJU. Whenever in operation, however, this piece of equipment will be dedicated to the specific needs of Neurological Sciences research, with the KCC investigator having limited access. The increased number of KCC investigators requiring high-speed cell sorting in their NIH-sponsored projects serves as the main justification for our request for a Cytomation MoFlo in the existing and operating KCC facility. Our application is focused on the need of this core facility to upgrade its services as a result of: 1) our NIH-investigators' increasing requests for desirable cell yields of sorted samples in reasonable time periods, which are currently unachievable with the present equipment; 2) an increasing demand for rare-event sorting within large cell populations, and for simultaneous analysis of greater than 4 fluorochrome-detectable parameters; and 3) the lack of sufficient and reliable access to near-by high speed sorting services. The availability of the Cytomation MoFlo instrument requested would allow outstanding basic and clinical research to be continued, and new projects to be initiated, at the KCC. It also would increase the efficiency of the existing Facility, allowing reliable use of the instruments by more investigators, and by other investigators (not in need of the machine requested here) to perform the more routine analysis and sorting in a timely manner on the old equipment, without the lag existent at present.

描述（由申请人提供）：该提案是要获得具有分类功能的最先进的流式细胞仪，以升级Kimmel癌症中心，Thomas Jefferson University的多用户流式细胞仪设施，从而最佳地支持NIH资助的癌症中心成员的研究活动。我们要求购买具有高速4路分类能力的MOFLO（TM）HTS超高速流式细胞仪，同时对大于4个荧光剂的常规分析以及单细胞沉积的增加能力。目前，KCC流式细胞仪设施是TJU校园这种类型的唯一设施。它配备了一个12岁的Coulter Epics Elite ESP，这也是一种分类工具，还有两个分析仪：4岁的Coulter Epics XL-MCL和15岁的Becton-Dickinson Facscalibur台式台式分析仪。在TJU的神经病学部门，有一个新近获得的BD ARIA分子（尽管尚未安装在新开发的设施中）。但是，每当运行时，这件设备都将致力于神经科学研究的特定需求，而KCC研究人员的访问有限。在其NIH赞助的项目中需要高速细胞分类的KCC调查人员数量增加，这是我们在现有和操作的KCC设施中要求MOFLO的要求的主要理由。我们的申请专注于该核心设施的需求，以升级以下原因：1）我们的NIH评估者在合理的时间段内提出了对分类样品所需的细胞收益率的不断增加的要求，目前的设备目前是无法实现的； 2）在大细胞群体中对稀有事件分类的需求不断增长，并同时分析了大于4个荧光色素可检测参数的需求； 3）缺乏足够可靠的访问近速排序服务。所需的MOFLO仪器的可用性将允许继续进行基本和临床研究，并在KCC上启动新项目。它还将提高现有设施的效率，从而允许更多调查人员以及其他调查人员（不需要此处要求的机器）对工具的可靠使用，以及时对旧设备进行及时进行更常规的分析和分类，而目前不存在lag。