CELL SORTER FACILITY

细胞分选设备

• 批准号: 3521497
• 负责人: BICE PERUSSIA
• 金额: $ 26.6万
• 依托单位: THOMAS JEFFERSON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-06-19 至 1993-06-18
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3521497
• 关键词: biomedical equipment purchase; biomedical facility; flow cytometry; immunofluorescence technique

This proposal is to establish a multi-user flow cytometry facility at the Jefferson Cancer Institute, to support the research activities of several members of the Department of Microbiology and Immunology and of that of Medicine. To this purpose, we request funds to purchase a Coulter EPICS Elite flow cytofluorimeter. Currently, Thomas Jefferson University does not possess a facility of this type nor a cell sorter/analyzer dedicated to research activities. Two analyzers exist on campus: an Epics-C in the Division of Medicine Oncology, and a FACScan in the Hospital Clinical Pathology Laboratories. The Epics-C is not an up-to-date instrument, can not satisfactorily perform multicolor analysis, lacks U.V. capabilities, and can not be updated. The FACScan is dedicated to the Clinical Laboratory and can be used for research activities only as time permits. Neither machine has cell sorting capabilities. Flow cytometry technology has rapidly progressed and several techniques required by the scientific projects of our group require the EPICS Elite instrument. This instrument will allow us to establish a multi-user facility and will provide it with the ability to perform simple immunofluorescence experiments, as well as to sort cells with high purity and velocity, to perform 3 and 4 color immunofluorescence analysis, efficient use of dyes requiring U.V. activation (e.g. Indo-1 and Hoechst 33342), and simultaneous analysis of cell cycle by DNA staining and surface antigens by two-color immunofluorescence. We propose to use the EPICS Elite for a variety of projects, including cellular immunology, hematology, control of cell growth, and tumor biology.

该建议是在 杰斐逊癌症研究所，以支持几个研究活动 微生物和免疫学系的成员以及 药品。 为此，我们要求资金购买Coulter Epics 精英流动细胞荧光仪。 目前，托马斯·杰斐逊大学（Thomas Jefferson University）确实 不具备这种类型的设施，也不拥有专用的细胞分类器/分析仪 研究活动。 校园中存在两个分析仪： 医学肿瘤科和医院临床的FACSCAN 病理实验室。 史诗-C不是最新的工具，可以 没有令人满意的多色分析，缺乏U.V.能力， 并且无法更新。 FACSCAN致力于临床 实验室，只能按时间许可来用于研究活动。 两种机器都没有单元格分类功能。 流式细胞仪技术 经过迅速发展，科学需要几种技术 我们小组的项目需要史诗精英工具。 这个乐器 将允许我们建立多用户设施，并将为其提供 执行简单免疫荧光实验的能力以及 要以高纯度和速度排序细胞，以执行3和4颜色 免疫荧光分析，有效使用U.V. 激活（例如Indo-1和Hoechst 33342），并同时分析 通过DNA染色和表面抗原的细胞周期通过两色 免疫荧光。 我们建议将史诗精英用于多种 项目，包括细胞免疫学，血液学，细胞控制 生长和肿瘤生物学。