Color-coded transposor-mediated rat mutagenesis

颜色编码转座子介导的大鼠诱变

• 批准号: 7077876
• 负责人: Richard R Behringer
• 金额: $ 17.59万
• 依托单位: UNIVERSITY OF TX MD ANDERSON CAN CTR
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-04-01 至 2008-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7077876
• 关键词: community; gene mutation; genes; genetics; genome; model; mutant; transposon /insertion element

DESCRIPTION (provided by applicant): Rats are very important laboratory animals for studying mammalian biology. These rodents are especially useful for physiological, neurobehavioral, organ transplantation, and toxicological studies. In addition, exceptional genetic and genomic resources are now available for the rat, including a genome that has been sequenced with a current assembly and annotation predicting 23,400 genes (www.ensembl.org/Rattus_norvegjcus) and more than 50,000 expressed sequence tags (ESTs). The rat model system is now primed for functional genomic studies. However, one particularly valuable resource to facilitate studies of gene function that is sorely lacking in the rat system is a large collection of mutants and a continuous source of new mutations. Although it is possible to routinely generate transgenic rats to express exogenous genes, there are no embryonic stem (ES) cells for this species that could be used to generate targeted mutations that is routine in the mouse. The ability to easily generate new mutant rat strains would significantly advance the use of this central laboratory animal for biomedical research. The primary objective of this proposal is to generate a Sleeping Beauty transposon mutagenesis system through transgenic rats to serve as a freely available resource to the research community to easily generate and molecularly identify new mutations in rats.

描述（由申请人提供）： 大鼠是研究哺乳动物生物学的非常重要的实验室动物。这些啮齿动物对于生理，神经行为，器官移植和毒理学研究特别有用。此外，现在可以为大鼠使用特殊的遗传和基因组资源，其中包括已对当前组装和注释进行测序的基因组，预测了23,400个基因（www.ensembl.org/rattus_norvegjcus）和超过50,000个表达序列标签（ESTS）。大鼠模型系统现在用于功能基因组研究。但是，在大鼠系统中严重缺乏基因功能研究的一种特别有价值的资源是大量突变体和连续的新突变来源。尽管可以常规地产生转基因大鼠表达外源基因，但是该物种的胚胎茎（ES）细胞没有可用于产生小鼠常规的靶向突变。轻松产生新的突变大鼠菌株的能力将显着提高这种中央实验室动物进行生物医学研究的使用。该提案的主要目的是通过转基因大鼠产生睡美人的转座子诱变系统，以作为研究界的自由使用资源，以轻松生成和分子鉴定大鼠的新突变。