CORE--BIOMOLECULAR RESEARCH CORE

核心--生物分子研究核心

• 批准号: 7550809
• 负责人: Jay William FOX
• 金额: $ 11.34万
• 依托单位: UNIVERSITY OF VIRGINIA
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-02-01 至 2008-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7550809
• 关键词: biochemistry; biomedical facility; chromatography; diabetes mellitus; mass spectrometry; microarray technology; molecular biology; oligonucleotides; phosphopeptides; polymerase chain reaction; posttranslational modifications; protein sequence; proteomics; two dimensional gel electrophoresis

The primary objectives of the Biomolecular Research Core of the Diabetes and Endocrinology Research Center (DERC) are to provide high quality, cost-effective, sophisticated analytical and synthetic services, instrumentation and consultation in the areas of protein, and DNA sciences. To achieve these goals the following specific services are offered: Protein Sciences - Amino acid analysis; Edman and mass spectrometric peptide sequencing and phosphopeptide sequencing; Mass spectrometric protein and peptide analysis and determination of post-translational protein modifications; CD spectropoladmetry; Dynamic light scattering; Large and small-scale peptide synthesis and anti-peptide antigen production. Proteomic analyses using 2D PAGE and orthogonai chromatography coupled with nanospray i/ms/ms. DNA Sciences - Automated DNA sequencing, genotyping and satellite mapping; DNA chemical manipulation. Affymetrix GeneChip and custom microarray gene profiling. Quantitative RT-PCR. High throughput oligonucleotide synthesis and "boutique" oligonucleotide synthesis. Expert consultation on the planning and implementation of research projects and individual experiments. These services are provided using either core personnel or the use of core instrumentation by trained investigators and students. Particular emphasis in the core is directed at the development of technologies and protocols which are in demand by investigators in the DERC. Recent expansion in the core includes a 2D gel electrophoresis service for sample preparation for mass spectrometric sequencing of proteins (proteomics), new mass spectrometers for ultra-high sensitivity protein sequence analysis, high throughput capillary DNA sequencer, high throughput oligonucleotide synthesizer; and a quantitative RT-PCR instrument for gene chip target validation and real-time PCR.

糖尿病和内分泌研究中心（DERC）生物分子研究核心的主要目标是在蛋白质和DNA科学领域提供高质量，成本效益，复杂的分析和合成服务，仪器和咨询。为了实现这些目标，提供了以下特定服务：蛋白质科学 - 氨基酸分析； Edman和质谱肽测序和磷酸肽测序；质谱蛋白和肽分析以及翻译后蛋白质修饰的测定； CD SpectroPopolAdmetry;动态光散射；大型和小型肽合成和抗肽抗原的产生。蛋白质组学分析使用2D页面和正交色谱法与纳米喷I/MS/MS结合。 DNA科学 - 自动DNA测序，基因分型和卫星映射； DNA化学 操纵。 Affymetrix Genechip和自定义微阵列基因分析。定量RT-PCR。高吞吐量寡核苷酸合成和“精品”寡核苷酸合成。有关研究项目和个人实验的计划和实施的专家咨询。 这些服务是使用核心人员或训练有素的研究人员和学生使用核心仪器提供的。核心中特别强调的是开发DERC研究人员需要的技术和协议。核心的最新扩展包括用于蛋白质质谱测序（蛋白质组学）的样品制备的2D凝胶电泳服务，新的质谱仪，用于超高灵敏度蛋白质序列分析，高吞吐量毛细血管DNA Sequencer，高吞吐量寡核苷酸合成器；以及用于基因芯片目标验证和实时PCR的定量RT-PCR仪器。