INSTRUMENTATION FOR PROTEOMICS

蛋白质组学仪器

• 批准号: 6292226
• 负责人: Jay William FOX
• 金额: $ 32.11万
• 依托单位: UNIVERSITY OF VIRGINIA
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-04-15 至 2002-04-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6292226
• 关键词: biomedical equipment purchase; gel electrophoresis; mass spectrometry

In this proposal, we are requesting support for the purchase of an instrument system to semi-automate sample processing for proteomic projects performed in the University of Virginia Biomolecular Research Facility for University investigators. Specifically, we are requesting a gel imaging system and associated software, gel spot picker, gel spot digester and digest spotter. Samples generated by these instruments will be subjected to automated mass mapping on our existing PE Voyager DE Pro MALDI-TOF instrument or if necessary, de novo sequencing with the facility's Finnigan LCQ ion-trap LC/MS/MS mass spectrometer. The overall goal of this program is to provide high throughput, low cost protein identification for investigators with proteomic related projects. Furthermore, this technology will be interfaced with the Microarray/GeneChip Program in the facility. The instruments will be housed in the University of Virginia Biomolecular Research Facility and will support the research proteomic projects of seven NIH-funded investigators. For these projects, we anticipate that the instruments will be in use approximately 80% of the available time. The remaining time will be available for new proteomic-related project development. There is no other mass spectrometric protein identification service, high throughput or otherwise, available at the University. Dr. Jay Fox, Director of the Biomolecular Research Facility, will be responsible for the overall management of the instrumentation. Dr. Nicholas Sherman will be responsible for mass spectrometry issues of proteomics projects and Dr. John Shannon will be responsible for the protein chemistry aspects of the proteomic projects. Both are staff members in the Biomolecular Research Facility. All three scientists will consult with investigators in the planning, execution and data analysis of proteomics projects. For the past seven years, the facility has offered mass spectrometric protein identification and de novo sequence analysis and no major difficulties are expected on the downstream end of sample analysis. A fee-for-service charge will be instituted to help defray the costs of staff salaries and supplies. Oversight of the Biomolecular Research Facility is provided by a standing committee appointed by the Dean of the Medical School. The Oversight Committee provides guidance and expertise directly to Dr. Fox for the management of the facility as well as complementing the scientific expertise of facility staff. One of the principal investigators in this proposal, Dr. Kevin Hazen, is a member of the committee. The University of Virginia Biomolecular Research Facility has been in operation for the past 20 years with continual fiscal support from the University of Virginia Health Sciences Center. The administration of the Health Sciences Center is committed to the long-term operation of the facility. This support, along with that of the Oversight Committee and facility staff, will serve to ensure adequate, appropriate instrument use for the fiscally sound and scientifically successful operation of instrumentation requested for the benefit of NIH-funded investigators at the University of Virginia..

在此提案中，我们要求支持购买仪器系统，以在弗吉尼亚大学生物分子研究机构为大学研究人员进行的蛋白质组学项目进行半自动样品处理。具体来说，我们要求凝胶成像系统和相关的软件，凝胶点拾取器，凝胶点消化器和Digest Spotter。这些仪器生成的样品将在我们现有的PE Voyager de Pro Maldi-Tof仪器上进行自动质量映射，或者在必要时，请与该设施的Finnigan LCQ离子陷阱LC/MS/MS/MS质谱仪进行从头测序。该计划的总体目标是为具有蛋白质组学相关项目的研究人员提供高通量，低成本的蛋白质识别。此外，这项技术将与该设施中的微阵列/Genechip计划联系起来。这些仪器将安置在弗吉尼亚大学生物分子研究机构中，并将支持七位NIH资助的研究人员的研究蛋白质组学项目。对于这些项目，我们预计该工具将使用约80％的可用时间。剩余时间将用于新的与蛋白质组学相关的项目开发。大学没有其他质谱蛋白识别服务，高吞吐量或其他大学。生物分子研究机构主管Jay Fox博士将负责该仪器的整体管理。 Nicholas Sherman博士将负责蛋白质组学项目的质谱问题，John Shannon博士将负责蛋白质组学项目的蛋白质化学方面。两者都是生物分子研究机构的工作人员。这三位科学家将在蛋白质组学项目的计划，执行和数据分析中与研究人员进行咨询。在过去的七年中，该设施提供了质谱蛋白鉴定和从头序列分析，并且在样本分析的下游端没有任何重大困难。将提出支付费用的费用，以帮助支付员工薪水和物资的费用。对生物分子研究机构的监督由医学院院长任命的常务委员会提供。监督委员会直接向Fox博士提供指导和专业知识，以管理该设施的管理，并补充了设施人员的科学专业知识。该提案中的主要调查员之一凯文·哈登（Kevin Hazen）博士是委员会的成员。弗吉尼亚大学生物分子研究机构在过去的20年中一直在运营，并在弗吉尼亚大学健康科学中心的持续财政支持下。卫生科学中心的管理致力于该设施的长期运作。这种支持，以及监督委员会和设施人员的支持，将有助于确保弗吉尼亚大学的NIH资助的调查人员受益的仪器的适当，适当的工具使用。