QUANTATIVE AND PHOSPHO PROTEOMICS INSTRUMENTATION

定量和磷酸化蛋白质组学仪器

• 批准号: 7335276
• 负责人: Jay William FOX
• 金额: $ 47.66万
• 依托单位: UNIVERSITY OF VIRGINIA
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-04-01 至 2007-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7335276
• 关键词: biomedical facility; dogs; proteins; proteomics; university

This subproject is one of many research subprojects utilizing the resources provided by a Shared Instrumentation Grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the grant, which is not necessarily the institution for the investigator. DESCRIPTION (provided by applicant): In this proposal, we are requesting support for the purchase of a mass spectrometer to perform experiments involving identification and quantification of protein expression levels and post-translational modifications (PTMs). Specifically, we are requesting a hybrid quadrupole time-of-flight mass spectrometer system (QSTAR XL) from Applied Biosystems. The abilities of this instrument to accurately measure both peptide masses and fragment ion masses as well as provide complementary MS/MS fragmentation patterns will offer increased certainty in the analysis of PTMs found in protein samples. In addition, the system has been engineered to facilitate not only the identification, but also the quantification of proteins within a given sample using novel iTRAQ reagents. As the field of proteomics moves beyond the level of simple identification into the more challenging realm of changes in protein structure and expression, it is vital that we acquire instrumentation which will allow our investigators to probe these effects. This instrument will be housed in the University of Virginia Biomolecular Research Facility and will support the research proteomics projects of eight NIH-funded investigators. For these projects, we anticipate that the instrument will be used approximately 80% of the available time. The remaining time will be made available for new proteomic project development. Dr. Jay Fox, Assistant Dean for Research, will be responsible for the overall management of the instrumentation. Dr. Nicholas Sherman will be responsible for interacting with investigators concerning the planning and execution of experiments and data interpretation. Dr. Kristina Nelson will be responsible for daily operation and maintenance of the instrument as well as data analysis. The Biomolecular Research Facility has been providing mass spectrometric expertise for the past 11 years, and we expect seamless integration of the requested system with current instrumentation. A fee-for-service charge will be instituted to help defray the costs of staff salaries and supplies. The oversight of the Biomolecular Research Facility is provided by a standing committee appointed by the Dean of the Medical School. The Oversight Committee provides guidance and expertise directly to Dr. Fox for the management of the facility as well as complementing the scientific expertise of facility staff. The University of Virginia Biomolecular Research Facility has been in operation for the past 24 years with continual fiscal support by the Health Sciences Center. The support by the administration, along with the operational plan, ensures adequate and appropriate instrument use for the fiscally sound and scientifically successful operation of instrumentation requested for the benefit of NIH-funded investigators at UVa.

该副本是利用NIH/NCRR资助的共享仪器赠款提供的资源的许多研究子项目之一。子弹和调查员（PI）可能已经从其他NIH来源获得了主要资金，因此可以在其他清晰的条目中代表。列出的机构是赠款，这不一定是调查人员的机构。描述（由申请人提供）：在此提案中，我们要求支持购买质谱仪，以执行涉及蛋白质表达水平和翻译后修饰（PTMS）的识别和量化的实验。具体而言，我们要求来自Applied Biosystems的混合四极飞机质谱仪系统（QSTAR XL）。该仪器的能力准确测量肽质量和片段离子块，以及提供互补的MS/MS片段化模式，将在蛋白质样品中发现的PTMS分析中增加确定性。此外，该系统已经过设计，不仅可以促进识别，还可以使用新型的ITRAQ试剂对给定样品中蛋白质进行定量。随着蛋白质组学领域超出了简单识别的水平，进入了蛋白质结构和表达变化的更具挑战性的领域，我们至关重要的是，我们获得仪器，这将使我们的研究人员能够探究这些影响。该工具将安置在弗吉尼亚大学生物分子研究机构中，并将支持八名NIH资助的研究人员的研究蛋白质组学项目。对于这些项目，我们预计该仪器将被使用约80％的可用时间。剩余时间将用于新的蛋白质组学项目开发​​。 研究副院长Jay Fox博士将负责该仪器的整体管理。尼古拉斯·谢尔曼（Nicholas Sherman）博士将负责与调查人员有关实验和数据解释的计划和执行。克里斯蒂娜·尼尔森（Kristina Nelson）博士将负责日常操作和维护仪器以及数据分析。在过去的11年中，生物分子研究机构一直在提供质谱专业知识，我们预计请求的系统与当前的仪器无缝整合。将提出支付费用的费用，以帮助支付员工薪水和物资的费用。 生物分子研究机构的监督由医学院院长任命的常务委员会提供。监督委员会直接向Fox博士提供指导和专业知识，以管理该设施的管理，并补充了设施人员的科学专业知识。弗吉尼亚大学生物分子研究机构在过去的24年中一直在运营，并得到了健康科学中心的持续财政支持。政府的支持以及运营计划，确保了对财政合理和科学成功的仪器的充分和适当的使用，要求其在UVA中获得NIH资助的研究人员的利益。