Comparative Analysis of Cell Death Regulation in Mosquitoes

蚊子细胞死亡调控的比较分析

• 批准号: 7141246
• 负责人: Lei Zhou
• 金额: $ 18.19万
• 依托单位: UNIVERSITY OF FLORIDA
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-06-15 至 2008-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7141246
• 关键词: Anopheles; Drosophilidae; cell death; genes; genome

DESCRIPTION (provided by applicant): Infectious diseases transmitted through mosquitoes account for millions of death world wide each year. Cell death (apoptosis) is an important component of host/vector immuno-response mechanism and has been implicated in the life cycle of pathogens in mosquitoes. However the understanding of cell death regulation in mosquito was hindered greatly by the fact that major insect cell death regulators, namely the reaper/hid/grim (RHG) like lAP-antagonists identified in Drosophila, were not present in the annotated mosquito (Anopheles gambia) genome. Using a systematic bioinformatics approach, we have identified several candidates of reaper/hid/grim-like lAP-antagonist in both the Anopheles and Aedes mosquito genomes. One of them, michelob_x has been characterized as indeed an lAP-antagonist. This proposed project aims at further characterizing these lAP-antagonists and their role in mediating cell death during mosquito development as well as in response to pathogen infection. Findings generated from this study should significantly improve our understanding of immuno-response and cell death regulation in mosquitoes. It is also quite possible that some of these genes, because of their tissue-ablation activity, can be used for mosquito population control strategies.

描述（由申请人提供）：通过蚊子传播的传染病每年导致全世界数百万人死亡。细胞死亡（细胞凋亡）是宿主/载体免疫应答机制的重要组成部分，并且与蚊子病原体的生命周期有关。然而，对蚊子细胞死亡调节的理解受到了很大的阻碍，因为主要的昆虫细胞死亡调节剂，即在果蝇中发现的类似 reaper/hid/grim (RHG) 的 lAP 拮抗剂，在注释的蚊子（冈比亚按蚊）中并不存在。 ）基因组。使用系统的生物信息学方法，我们在按蚊和伊蚊基因组中鉴定了几种 reaper/hid/grim 样 lAP 拮抗剂的候选物。其中一个，michelob_x 已被描述为确实是 lAP 拮抗剂。该项目旨在进一步表征这些 lAP 拮抗剂及其在蚊子发育过程中介导细胞死亡以及响应病原体感染中的作用。这项研究的结果应该会显着提高我们对蚊子免疫反应和细胞死亡调节的理解。其中一些基因也很可能由于其组织消融活性而可用于蚊子种群控制策略。