A COMBINED COMPUTATIONAL AND EXPERIMENTAL STUDY ON THE LIGAND-GATING IN CNG AND

CNG 和配体门控的综合计算和实验研究

• 批准号: 7723187
• 负责人: Lei Zhou
• 金额: $ 0.05万
• 依托单位: CARNEGIE-MELLON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-08-01 至 2009-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7723187
• 关键词: Address; Computer Retrieval of Information on Scientific Projects Database; Data; Electrostatics; Funding; Grant; Institution; Ligands; Proteins; Published Comment; Research; Research Personnel; Resources; Solvents; Source; United States National Institutes of Health; improved; research study; response; size

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We appreciate the comments on the renewal application for MRAC MCB060027, A combined computational and experimental study on the ligand - gating in CNG and HCN channels, by Lei Zhou and Steven A. Siegelbaum. We found that most of the comments from both anonymous reviewers were very constructive and helpful for us to further improve the original proposal. As quoted in the Proposal Comments by Allocation Staff, one of the major technical concerns by reviewer 1 is on the weakness in the application of TI. Actually we had carried out the TI almost exactly as described by reviewer 1 (first removing electrostatic and then VDW interactions; comparing the difference between removing ligand from protein and versus removing it from bulk solvent). However, we were concerned with the file size (already 15 pages) so that we didnt present all the technical details or preliminary results in the renewal application. Here we carefully address concerns raised by both reviewers and add those technical details together with the related preliminary data. We hope that this response together with the original renewal application will satisfy these concerns and enable this proposal to be found acceptable so that we may continue the research proposed in this MRAC renewal application. Thank you for your consideration.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此，可以在其他清晰的条目中表示。列出的机构是 对于中心，这不一定是调查员的机构。 我们感谢Lei Zhou和Steven A. Siegelbaum对MRAC MCB060027的更新应用的评论，该评论是对CNG和HCN通道中配体 - CNG和HCN通道中配体 - 门控的​​合并研究的评论。我们发现，两位匿名审稿人的大多数评论都非常有建设性和有用，以进一步改善原始建议。正如分配工作人员的提案评论中引用的那样，审稿人1的主要技术问题之一是TI的应用弱点。实际上，我们几乎按照审稿人1的描述（首先去除静电和VDW相互作用；比较从蛋白质中去除配体与从散装溶剂中取出的差异之间的差异）。但是，我们关注文件大小（已经15页），因此我们没有在续签应用程序中介绍所有技术细节或初步结果。在这里，我们仔细解决了审阅者提出的问题，并将这些技术细节以及相关的初步数据添加。我们希望这种响应以及最初的续订申请能够满足这些关注，并使该提案被发现可以接受，以便我们可以继续在此MRAC更新申请中提出的研究。谢谢您的考虑。