INFRASTRUCTURE 1: CORE RESEARCH LABORATORIES

基础设施 1：核心研究实验室

• 批准号: 7336050
• 负责人: JOHN B WILLIAMS
• 金额: $ 4.56万
• 依托单位: TUSKEGEE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-06-01 至 2007-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7336050
• 关键词: INFRASTRUCTURE; CORE; RESEARCH; LABORATORIES

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The CBR/RCMI Core Facility enhances multidisciplinary research of Tuskegee University investigators in Microscopy/Imaging, Flow Cytometry, Cell Culture and Molecular Biology, and Bioinformatics and Computational Biology. This year, the Core Facility has provided service and assistance to 16 users representing 3 academic colleges and 9 scientific disciplines. These investigators published or submitted 5 peer reviewed papers and presented 9 papers or posters. The Imaging Facility continues to offer service to the largest number of investigators while the Cell Culture and Molecular Biology facilities accumulated the most "man hours" of use. . A Q Imaging CE digital camera operating through C Imaging Systems Simple PCI software was recently added to the light imaging facility. This camera greatly enhances the fluorescent microscopy capabilities of the facility and should allow the recording of chemoluminescent gels without the use of radiographic film. The Philips 400 TEM was equipped with a Scientific Instruments & Applications (SIA) Pelter Cooled CCD Camera (3.2 megapixels); images captured using the SIA software package (TIFF files) are processed and analyzed. Molecular biology capabilities have been significantly enhanced through the purchase of several state of the art instruments. These include a BIO-TEK SYNERGY HT microplate reader, which reads 6-to-384 well plates and performs fluorescence, absorbance and luminescence measurements. An Eppendorf Mastercycler has been added updating conventional PCR. A Cepheid Smartcycler 2 has been purchased and provides outstanding Real-time PCR capabilities. The instrument employs 4 optical channels; emissions range from 510 nm to 750 nm. These channels permit simultaneous detection of four "probes". The instrument provides real-time display of growth curves, temperature profiles, and melting curves with qualitative, quantitative, and growth curve analysis. The goal of the Bioinformatics and Computational Biology Facility is to increase participation of faculty in bioinformatics and computational biology research through the use of web based infrastructure, especially in genomics and proteomics. We have added updated software from Accelrys and are continuing our association with the University of Alabama at Birmingham Genomics Core Facility.

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子弹和调查员（PI）可能已经从其他NIH来源获得了主要资金，因此可以在其他清晰的条目中代表。列出的机构适用于该中心，这不一定是调查员的机构。 CBR/RCMI核心设施增强了Tuskegee University研究者在显微镜/成像，流式细胞仪，细胞培养和分子生物学以及生物信息学和计算生物学方面的多学科研究。今年，核心设施为16位代表3所学院和9个科学学科的用户提供了服务和帮助。这些调查人员发表或提交了5份同行评审的论文，并介绍了9篇论文或海报。成像设施继续为研究人员提供服务，而细胞培养和分子生物学设施积累了最“人的小时”。 。 Q成像CE CE通过C成像系统运行的数码相机最近被添加到轻型成像设施中。该摄像机大大增强了设施的荧光显微镜功能，并应允许在不使用放射线膜的情况下记录化学发光凝胶。飞利浦400 TEM配备了科学仪器和应用程序（SIA）Pelter冷却CCD摄像头（3.2百万像素）；处理和分析使用SIA软件包（TIFF文件）捕获的图像。通过购买几种最先进的工具，分子生物学能力得到了显着增强。其中包括生物技术协同型HT微板读取器，该读取器读取6至384个井板并进行荧光，吸光度和发光测量值。 Eppendorf Mastercycler已添加更新常规PCR。已购买了Cepheid SmartCycler 2，并提供出色的实时PCR功能。该仪器采用4个光通道；排放范围从510 nm到750 nm。这些通道允许同时检测四个“探针”。该仪器通过定性，定量和生长曲线分析提供实时显示生长曲线，温度曲线和熔解曲线。生物信息学和计算生物学设施的目的是通过使用基于Web的基础架构，尤其是基因组学和蛋白质组学来增加教师在生物信息学和计算生物学研究中的参与。我们添加了Accelrys的更新软件，并正在继续与伯明翰基因组核心设施的阿拉巴马大学联系。