INFRASTRUCTURE 1: CORE RESEARCH LABORATORIES

基础设施 1：核心研究实验室

• 批准号: 7959269
• 负责人: JOHN B WILLIAMS
• 金额: $ 18.32万
• 依托单位: TUSKEGEE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-09-18 至 2010-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7959269
• 关键词: Animals; Biology; Biomedical Research; Cell Culture Techniques; Cell Separation; Cells; Chemistry; Computer Retrieval of Information on Scientific Projects Database; Computer software; Computers; Core Facility; Coupled; Digital Photography; Discipline; Equipment; Faculty; Flow Cytometry; Fluorescence Microscopy; Fluorescent Probes; Funding; Gel; Grant; Hour; Image; Image Analysis; Imagery; Institution; Laboratories; Laboratory Research; Life; Methodology; Microscope; Molecular Biology; Operative Surgical Procedures; Plants; Plug-in; Recruitment Activity; Reindeer; Research; Research Infrastructure; Research Personnel; Research Project Grants; Resources; Running; Science; Series; Services; Source; Specimen; System; Time; United States National Institutes of Health; Universities; Veterinary Medicine; college; digital; digital imaging; fluorescence microscope; fovea centralis; image processing; instrument; laboratory facility; prospective; research study; tool

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The services and methodologies provided to the Tuskegee University research faculty by the CORE I Research Laboratories significantly enhance their research capabilities. Current and prospective users, about 21 (the number varies as new faculty are recruited), represent 3 academic colleges and diverse scientific disciplines. 1. Imaging Facility: The facility provides EM, LM and gel imaging and analysis. Digital images are captured with a Kodak 290 used as Photoshop plug in or a Q Imaging CE camera operating through C Imaging Systems Simple PCI software (or as a Photoshop plug in) or MetaMorph software. Images are processed using Photoshop 7.0 or CS2 and analyzed with Fovea Pro 3 Image Analysis Tool Kit (Reindeer Graphics), C Imaging Systems Simple PCI 6, or MetaMorph software. Gels are analyzed with Kodak EDAS 290 software. Operations are run on Macintosh or Gateway E Series (Windows XP) computers. Fluorescence microscopy, has been greatly enhanced by the addition of a Leica DM 5000B (upright microscope) coupled to the existing Q Imaging CE digital camera (operating through C Imaging Systems Simple PCI software). This system allows the visualization and digital capturing of images of specimens tagged with multiple fluorescent probes and the fusion of the multiple images into a single photograph. Additionally, a Leica DM IRE2 (inverted fluorescent microscope) equipped with a cell incubation chamber has been acquired. This system is also equipped with a similar digital photography system and will allow the visualization of live cell experiments over extended time periods. These instruments were obtained with funds provided by CBR/RCMI and the NIH Export Grant (#3P20MD000195-05S1). This facility is used by the most investigators and has provided services to researchers from diverse disciplines including: Animal Science, Plant Science, Biology, Chemistry, Veterinary Biomedical Sciences and Pathobiology, and Material Sciences. 2 Cell Sorting / Flow Cytometry Facility: The Center acquired an Accuri C6 Flow Cytometer System. This system is administered by Dr. Clayton Yates and is primarily used by investigators from Biology and Veterinary Medicine. 3. Cell Culture and Molecular Biology Laboratories: These facilities provide laboratory space and molecular biology equipment to CBR/RCMI and other biomedical investigators. These laboratories easily receive the most "man-hours" of use of any of the CBR/RCMI CORE Facilities. Drs. T. Turner (CBR/RCMI funded project), R. Troy, M. Martinez, and C. Yates's (Non- CBR/RCMI funded projects) research teams use the facilities and equipment daily.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 由核心I研究实验室提供给Tuskegee University研究学院的服务和方法可显着增强其研究能力。 当前和潜在的用户大约21个（随着新教师的招募而变化），代表了3所学术学院和多样化的科学学科。 1。成像设施：该设施提供EM，LM和凝胶成像和分析。 数字图像用用作Photoshop插件的Kodak 290捕获，或通过C成像系统操作的Q Imaging CE摄像头简单PCI软件（或Photoshop插件）或Metamorph软件。 使用Photoshop 7.0或CS2处理图像，并使用Fovea Pro 3图像分析工具套件（Reindeer Graphics），C成像系统简单PCI 6或Metamorph软件进行分析。 用柯达EDAS 290软件分析凝胶。 操作是在Macintosh或Gateway E系列（Windows XP）计算机上运行的。 通过添加与现有Q成像CE CE数码相机（通过C成像系统操作简单PCI软件运行），荧光显微镜大大增强了荧光显微镜。 该系统允许可视化和数字捕获用多个荧光探针标记的标本的图像，并将多个图像融合到一张照片中。 此外，已经采集了配备了细胞孵育室的Leica DM IRE2（倒荧光显微镜）。 该系统还配备了类似的数字摄影系统，并将允许在延长时间段内可视化实时细胞实验。 这些工具是通过CBR/RCMI和NIH出口赠款（＃3P20MD000195-05S1）提供的资金获得的。 大多数研究人员使用了该设施，并为来自不同学科的研究人员提供了服务，包括：动物科学，植物科学，生物学，化学，兽医生物医学科学和病理生物学以及物质科学。 2细胞分类 /流式细胞仪设施：中心获得了精确的C6流式细胞仪系统。 该系统由Clayton Yates博士管理，主要由生物学和兽医医学的研究人员使用。 3。细胞培养和分子生物学实验室：这些设施为CBR/RCMI和其他生物医学研究者提供实验室空间和分子生物学设备。 这些实验室很容易获得使用任何CBR/RCMI核心设施的最“工时”。 博士。 T. Turner（CBR/RCMI资助的项目），R。Troy，M。Martinez和C. Yates（非CBR/RCMI资助的项目）研究团队每天使用设施和设备。