OPTICAL IMAGING FACILITY

光学成像设备

• 批准号: 7335998
• 负责人: PRISCILA SANABRIA
• 金额: $ 10.5万
• 依托单位: UNIVERSIDAD CENTRAL DEL CARIBE
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-08-01 至 2007-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7335998
• 关键词: OPTICAL; IMAGING; FACILITY

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. As part of a previous funded RCMI cycle, an Optical Imaging Facility was established at UCC during the fall of 1999. The first major equipment acquired for this facility was a sensitive microscope-based system that allows the assessment of cellular responses such as calcium signaling with fast temporal resolution. The system also allows the spatial assessment of this functional variable, which means that an investigator is able to identify the heterogeneity of calcium signal responses in neighboring groups of cells. This proposal requests funds to continue providing technical support for this time-lapsed cell imaging system and the maintenance of its sophisticated equipment. Due to the nature of experiments, this particular microscope setup is a highly dedicated system. Therefore, in order to provide technical support for other microscope-based applications, this proposal is also requesting funds to support the integration of an immunocytochemistry laboratory into the Optical Imaging Facility. The immunocytochemistry laboratory specializes in the qualitative identification and localization of cells bearing selective markers by employing specific antibodies to these molecules. The referenced laboratory was organized in 1999 through the initiatives of Drs. Serguei Skatchkov and Misty Eaton, who have been successfully using this technology in the experimental design of their projects. In 1997, Dr. Skatchkov, thanks to an earlier RCMI supported Methodology Transfer Program, received training in a leading immunocytochemistry laboratory at Humboldt University, Berlin. The immunocytochemistry laboratory equipment includes an upright fluorescence microscope and a high-resolution digital camera. Immunocytochemistry data obtained in this laboratory has been published in peer-reviewed journals and presented in local and national symposiums. Currently, there are several projects that will benefit directly from the service provided by this microscope-based technology. Therefore, the aims of the Optical Imaging Facility are:1. To provide technical support for the time and spatial resolution imaging systems.2. To integrate an immunocytochemistry laboratory into the Optical Imaging Facility.3. To disseminate in our academic community the capabilities of our facility.4. To work on the submission of a grant for a confocal microscope.The continued support of the Optical Imaging Facility will constitute an asset for the Cell and Molecular Biology Center since it will contribute to enhance the productivity of its current participants and will constitute an attractive research resource for potential new investigators/ collaborators. The immunocytochemisty capabilities at the Optical Imaging Facility will complement our existing imaging platform that is configured for the rapid acquisition and analysis of fluorescently tagged reporters of intracellular calcium.

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子弹和调查员（PI）可能已经从其他NIH来源获得了主要资金，因此可以在其他清晰的条目中代表。列出的机构适用于该中心，这不一定是调查员的机构。作为先前资助的RCMI循环的一部分，在1999年秋天在UCC建立了光学成像设施。该设施获得的第一个主要设备是基于敏感显微镜的系统，该系统允许评估细胞反应，例如钙信号，例如钙信号传导，快速的时间分辨率。该系统还允许对该功能变量进行空间评估，这意味着研究者能够识别邻近细胞组中钙信号反应的异质性。该提案要求资金继续为该时间贴上的单元成像系统提供技术支持，并维护其复杂的设备。由于实验的性质，这种特殊的显微镜设置是一个高度专用的系统。因此，为了为其他基于显微镜的应用提供技术支持，该提案还要求资金支持将免疫细胞化学实验室集成到光学成像设施中。免疫细胞化学实验室通过采用对这些分子的特定抗体来专门研究具有选择性标记的细胞的定性鉴定和定位。引用的实验室由DRS的倡议于1999年组织。 Serguei Skatchkov和Misty Eaton，他们在项目的实验设计中成功使用了这项技术。 1997年，由于较早的RCMI支持方法转移计划，Skatchkov博士接受了柏林洪堡大学领先的免疫细胞化学实验室的培训。 免疫细胞化学实验室设备包括直立的荧光显微镜和高分辨率数码相机。该实验室获得的免疫细胞化学数据已在同行评审期刊上发表，并在本地和国家研讨会上介绍。当前，有几个项目将直接从该基于显微镜的技术提供的服务中受益。因此，光学成像设施的目的是：1。为时间和空间分辨率成像系统提供技术支持2。将免疫细胞化学实验室整合到光学成像设施中。3。为了在我们的学术界传播我们设施的能力4。致力于提交共聚焦显微镜的赠款。光学成像设施的持续支持将构成细胞和分子生物学中心的资产，因为它将有助于提高其当前参与者的生产率，并构成一项有吸引力的研究潜在的新调查人员/合作者资源。光学成像设施的免疫细胞化学能力将补充我们现有的成像平台，该平台配置为快速获取和分析细胞内钙的荧光标记的记者。