OPTICAL IMAGING FACILITY

光学成像设备

• 批准号: 7561496
• 负责人: PRISCILA SANABRIA
• 金额: $ 11.68万
• 依托单位: UNIVERSIDAD CENTRAL DEL CARIBE
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-08-01 至 2008-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7561496
• 关键词: Antibodies; Berlin; Calcium; Calcium Signaling; Cells; Communities; Complement; Computer Retrieval of Information on Scientific Projects Database; Data; Equipment; Experimental Designs; Funding; Grant; Heterogeneity; Image; Institution; Journals; Laboratories; Maintenance; Methodology; Microscope; Molecular Biology; Nature; Participant; Peer Review; Productivity; Publishing; Reporter; Request for Proposals; Research; Research Personnel; Resolution; Resources; Services; Source; System; Technology; Time; Training; United States National Institutes of Health; Universities; Work; base; cellular imaging; digital; falls; fluorescence microscope; immunocytochemistry; optical imaging; programs; research study; response; symposium

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. As part of a previous funded RCMI cycle, an Optical Imaging Facility was established at UCC during the fall of 1999. The first major equipment acquired for this facility was a sensitive microscope-based system that allows the assessment of cellular responses such as calcium signaling with fast temporal resolution. The system also allows the spatial assessment of this functional variable, which means that an investigator is able to identify the heterogeneity of calcium signal responses in neighboring groups of cells. This proposal requests funds to continue providing technical support for this time-lapsed cell imaging system and the maintenance of its sophisticated equipment. Due to the nature of experiments, this particular microscope setup is a highly dedicated system. Therefore, in order to provide technical support for other microscope-based applications, this proposal is also requesting funds to support the integration of an immunocytochemistry laboratory into the Optical Imaging Facility. The immunocytochemistry laboratory specializes in the qualitative identification and localization of cells bearing selective markers by employing specific antibodies to these molecules. The referenced laboratory was organized in 1999 through the initiatives of Drs. Serguei Skatchkov and Misty Eaton, who have been successfully using this technology in the experimental design of their projects. In 1997, Dr. Skatchkov, thanks to an earlier RCMI supported Methodology Transfer Program, received training in a leading immunocytochemistry laboratory at Humboldt University, Berlin. The immunocytochemistry laboratory equipment includes an upright fluorescence microscope and a high-resolution digital camera. Immunocytochemistry data obtained in this laboratory has been published in peer-reviewed journals and presented in local and national symposiums. Currently, there are several projects that will benefit directly from the service provided by this microscope-based technology. Therefore, the aims of the Optical Imaging Facility are:1. To provide technical support for the time and spatial resolution imaging systems.2. To integrate an immunocytochemistry laboratory into the Optical Imaging Facility.3. To disseminate in our academic community the capabilities of our facility.4. To work on the submission of a grant for a confocal microscope.The continued support of the Optical Imaging Facility will constitute an asset for the Cell and Molecular Biology Center since it will contribute to enhance the productivity of its current participants and will constitute an attractive research resource for potential new investigators/ collaborators. The immunocytochemisty capabilities at the Optical Imaging Facility will complement our existing imaging platform that is configured for the rapid acquisition and analysis of fluorescently tagged reporters of intracellular calcium.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目及 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 作为先前资助的 RCMI 周期的一部分，UCC 于 1999 年秋季建立了光学成像设施。为该设施购买的第一个主要设备是基于灵敏显微镜的系统，该系统可以评估细胞反应，例如钙信号传导快速时间分辨率。 该系统还允许对该功能变量进行空间评估，这意味着研究人员能够识别邻近细胞群中钙信号反应的异质性。 该提案要求资金继续为这种延时细胞成像系统及其精密设备的维护提供技术支持。 由于实验的性质，这种特殊的显微镜设置是一个高度专用的系统。 因此，为了为其他基于显微镜的应用提供技术支持，该提案还请求资金支持将免疫细胞化学实验室整合到光学成像设施中。 免疫细胞化学实验室专门通过使用针对这些分子的特异性抗体来定性识别和定位带有选择性标记的细胞。参考实验室是在 Drs. 的倡议下于 1999 年组建的。 Serguei Skatchkov 和 Misty Eaton 已在其项目的实验设计中成功地使用了这项技术。 1997 年，Skatchkov 博士得益于早期 RCMI 支持的方法学转移计划，在柏林洪堡大学领先的免疫细胞化学实验室接受了培训。 免疫细胞化学实验室设备包括正置荧光显微镜和高分辨率数码相机。该实验室获得的免疫细胞化学数据已发表在同行评审期刊上，并在地方和国家研讨会上发表。 目前，有几个项目将直接受益于这种基于显微镜的技术提供的服务。 因此，光学成像设备的目标是： 1． 为时间和空间分辨率成像系统提供技术支持； 2． 将免疫细胞化学实验室整合到光学成像设施中。3． 在我们的学术界传播我们设施的能力。4。 致力于提交共焦显微镜拨款。光学成像设施的持续支持将成为细胞和分子生物学中心的一项资产，因为它将有助于提高现有参与者的生产力，并将构成一项有吸引力的研究潜在新研究人员/合作者的资源。 光学成像设施的免疫细胞化学能力将补充我们现有的成像平台，该平台配置用于快速采集和分析细胞内钙的荧光标记报告基因。