Basic Science Core - Imaging

基础科学核心 - 成像

基本信息

批准号：
10588228
负责人：
Deepak Kaushal
金额：
$ 13.33万
依托单位：
TEXAS BIOMEDICAL RESEARCH INSTITUTE
依托单位国家：
美国
项目类别：
财政年份：
2022
资助国家：
美国
起止时间：
2022-03-10 至 2027-02-28
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10588228
关键词：
Address Animal Model Animals Basic Science Biology Biomedical Research Caring Cell Separation Cells Cellular Immunology Cities Clinical Clinical Management Clinical Services Collaborations Communication Communities Complement Computational algorithm Computer Analysis Confocal Microscopy Containment Data Disadvantaged Discipline Educational Curriculum Equipment Evaluation Experimental Designs Flow Cytometry Fluorescent in Situ Hybridization Fluorochrome Freezing Funding Funding Agency Genomics Goals Health Professional Image Image Analysis Immunofluorescence Immunologic Inpatients Institution Knowledge Label Laboratories Lasers Leadership Learning Light Magnetic Resonance Imaging Mexico Microscopy Molecular Optics Outpatients Paraffin Participant Positron-Emission Tomography Primates Publishing RNA Recording of previous events Research Research Institute Research Personnel Resources Robotics Rodent Science Scientist Services Sorting Structure System Techniques Testing Texas Thick Tissues Training Training Programs Tuberculosis United States National Institutes of Health Work X-Ray Computed Tomography animal safety biocontainment facility cohort confocal imaging cytate design experience high dimensionality imaging capabilities imaging modality imaging platform imaging study in vivo imaging system live cell imaging microscopic imaging multidisciplinary next generation optical imaging peer prevent programs prospective skills tomography tool whole animal imaging

项目摘要

TEXAS BIOMED IN-TRAC Research Imaging Core ABSTRACT The Research Imaging Core will leverage key strengths of Texas Biomed, namely a strong cohort of NIH- funded TB investigators with a history of collaboration and with experience in different imaging platforms. IN- TRAC participants will benefit from access to unique NHP resources in the Southwest National Primate Research Center (SNPRC) and its cutting edge imaging capabilities. The Research Imaging Core will also establish an education curriculum to introduce IN-TRAC participants to a variety of cell-based and whole animal imaging platforms. AIM 1: Provide didactic and hands-on training program in confocal microscopy and optical imaging. IN-TRAC participants will learn multi-label immunofluorescence or fluorescence in-situ hybridization (FISH) confocal microscopy, which allows for 3- or 4-D evaluation of tissues and cells with multiple fluorochrome labels. AIM 2. Provide didactic and hands-on training in cutting-edge cellular immunology techniques requiring imaging support or computational analysis. The Texas Biomed Biology, Microscopy and Molecular Cores provide high-dimensional, multi-parameter flow cytometry and analysis as well as BSL3 sorting capabilities. Established TB researchers will provide training live cell imaging (Cytation 5) and scRNAseq (10x Genomics) in BSL3. AIM 3: Provide didactic and hands-on training in live imaging using PET/CT and IVIS systems. Training in whole-body live Positron Emission Tomography (PET) and X-ray Computed Tomography (CT) imaging of NHP and in-vivo imaging system (IVIS) of rodents within the BSL3. IN-TRAC participants will extern at the NHP MRI facilities at UTHSCA to learn the advantages and limitations of this imaging platform. At the completion of the Research Imaging Core, participants will be able to effectively interpret published data that use imaging modalities for NHP, rodents, tissues, cells and RNA; design experiments to test their own hypothesis; and participate in TB research that uses the appropriate animal model.

德克萨斯州生物轨迹内研究成像核心摘要研究成像核心将利用德克萨斯州生物元素的关键优势，即NIH-的强大队列资助的结核病研究人员具有协作历史，并具有不同的成像平台的经验。在- TRAC参与者将受益于在西南国家灵长类动物研究中获得独特的NHP资源中心（SNPRC）及其前沿成像功能。研究成像核心还将建立教育课程将跟踪参与者引入各种基于细胞和整个动物成像平台。 AIM 1：在共聚焦显微镜和光学上提供教学和动手培训计划成像。 trac参与者将学习多标签免疫荧光或荧光原位杂交（FISH）共聚焦显微镜，可以对具有多个荧光色体的组织和细胞进行3或4-D评估标签。 AIM 2。在尖端的细胞免疫学技术中提供教学和动手培训需要成像支持或计算分析。德克萨斯生物学生物学，显微镜和分子核心提供高维，多参数流式细胞仪和分析以及BSL3排序功能。成熟的结核病研究人员将提供训练活细胞成像（Cytation 5）和Scrnaseq（10倍）基因组学）在BSL3中。 AIM 3：使用PET/CT和IVI提供实时成像的教学和动手培训系统。全身活性正电子发射断层扫描（PET）和X射线计算机断层扫描中的培训（CT）BSL3中啮齿动物的NHP和体内成像系统（IVI）的成像。牵引力参与者将在UTHSCA的NHP MRI设施中进行外观，以了解此成像平台的优势和局限性。在研究成像核心的完成，参与者将能够有效地解释已发布的数据对NHP，啮齿动物，组织，细胞和RNA使用成像方式；设计实验以测试自己的假设;并参与使用适当动物模型的结核病研究。