Efferent Innervation of Identified Vestibular Afferents

已识别的前庭传入神经的传出神经支配

• 批准号: 7150119
• 负责人: ANNA LYSAKOWSKI
• 金额: $ 40.05万
• 依托单位: UNIVERSITY OF ILLINOIS AT CHICAGO
• 依托单位国家: 美国
• 财政年份: 1995
• 资助国家: 美国
• 起止时间: 1995-08-01 至 2009-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7150119
• 关键词: acetylcholine; adenosine triphosphate; afferent nerve; brain stem; calcitonin gene related peptide; calretinin; chinchilla; confocal scanning microscopy; efferent nerve; enkephalins; immunocytochemistry; immunoelectron microscopy; innervation; muscarinic receptor; neuroanatomy; neurochemistry; neuronal transport; nitric oxide synthase; polymerase chain reaction; vestibular apparatus; western blottings

DESCRIPTION (provided by applicant): The purpose of this grant is to investigate the efferent innervation of the peripheral vestibular apparatus. Knowledge of the neurotransmitters and projections from brainstem efferent neurons to the peripheral labyrinth will help us to understand the role of this system in health and disease. We build on our past work suggesting that vestibular efferent neurons are heterogeneous in their transmitter composition and peripheral targets. Such heterogeneity challenges our long-held view that vestibular efferents are a nonspecific system. Experiments are done in the chinchilla and will concentrate on the peripheral organization of the efferent system. Anatomical, as well as immunohistochemical and other molecular approaches, are used to study the structural organization of the efferent system with both confocal and electron microscopy. Efferent neurons in the periphery are identified by means of anterograde tracer injections into their brainstem nuclei of origin and their distinctive synaptic profiles in the periphery. Afferent classes are identified by means of specific immunohistochemical markers (calretinin, peripherin). Efferent structure is correlated with hair cell and afferent classes and with the regional organization of the cristae and maculae. Specific aims are: 1) to determine by anterograde tracing the projection patterns and terminal fields of ipsilaterally-, contralaterally-, and bilaterally-projecting efferent neurons in the cristae and maculae: 2) to specify nicotinic. muscarinic and CGRP receptor subtypes on hair cells and afferents: and 3) to examine subcellular mechanisms of efferent action, specifically the localization of calcium-related pumps and channels in subsynaptic cisterns. The intent is to produce a body of morphological, neurochemical and cell biological knowledge from which physiologically and pharmacologically testable hypotheses can be derived.

描述（由申请人提供）：该赠款的目的是研究周围前庭设备的传出神经。对神经递质的知识以及从脑干源像神经元到周围迷宫的预测将有助于我们了解该系统在健康和疾病中的作用。我们以过去的工作为基础，表明前庭传出神经元在发射机组成和外围靶标的异质性。这种异质性挑战了我们长期以来的观点，即前庭产物是一种非特异性系统。实验是在龙猫进行的，将集中于传出系统的外围组织。解剖学以及免疫组织化学方法和其他分子方法用于研究具有共聚焦和电子显微镜的传出系统的结构组织。外围神经元的传出神经元是通过向周围脑干核和周围独特的突触特征注射到其脑干核中鉴定的。传入类别通过特定的免疫组织化学标记（钙蛋白素，外周）鉴定。传出结构与毛细胞和传入类别以及Cristae和Maculae的区域组织相关。具体目的是：1）确定同侧，外侧和双侧射击神经元的投射模式和末端场的投影模式和末端场：指定尼古丁。毛细胞和传入的毒蕈碱和CGRP受体亚型：和3）检查传出作用的亚细胞机制，特别是钙相关泵和突触下蓄水池中钙相关的泵和通道的定位。目的是产生形态学，神经化学和细胞生物学知识的体系，可以从生理和药理上可检验的假设得出这些知识。