A STUDY OF THE STRIATED ORGANELLE IN VESTIBULAR ENDORGANS

前庭恩多根条纹细胞器的研究

• 批准号: 7957639
• 负责人: ANNA LYSAKOWSKI
• 金额: $ 1.56万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-06-15 至 2010-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7957639
• 关键词: Apical; Architecture; Auditory; Cell physiology; Cells; Computer Retrieval of Information on Scientific Projects Database; Funding; Goals; Grant; Hair Cells; Image Analysis; Institution; Knowledge; Membrane Potentials; Modeling; Organelles; Research; Research Personnel; Resources; Sensory; Source; Stereocilium; Structure; Subcellular structure; United States National Institutes of Health; Variant; Vestibular Hair Cells; Vestibular Labyrinth; response; three dimensional structure

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. In the mammalian vestibular labyrinth, hair cells are capable of generating axial deformations in response to variations in transmembrane potential. Our understanding of electromechanical transduction in the hair cells depends on precise knowledge of intracellular structure, and efforts to model hair cell physiology are likewise limited to the fidelity of known intracellular geometry. This study focuses on the ultrastructure of vestibular hair cells. More precisely, we are looking at the subcuticular region (below the cuticular plate), trying to visualize and analyze rootlet architecture at the junction of the stereocilia bundle and apical region of the cell. Our main concern is the striated organelle (STO), a unique structure located at the apical end of hair cells (auditory and vestibular sensory cells), just below the cuticular plate. It is particularly prominent in mammalian type I vestibular hair cells. The central hypothesis of this proposal is that striated organelles provide structural and functional connections between the apical and basal parts of the hair cell. The goals of this proposal are to describe the three-dimensional structure of this particular organelle - Aim 1 - and to identify its components (or at least, some of the components) - Aim 2.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 在哺乳动物的前庭迷宫中，毛细胞能够响应跨膜电位的变化而产生轴向变形。我们对毛细胞中机电转导的理解取决于对细胞内结构的精确知识，并且对毛细胞生理的努力也限于已知细胞内几何形状的忠诚度。这项研究的重点是前庭毛细胞的超微结构。更确切地说，我们正在查看下部区域（在表皮板下方），试图可视化和分析细胞立体束和顶端区域的连接处的根架结构。我们的主要关注点是横纹器（STO），这是一个位于毛细胞顶端（听觉和前庭感觉细胞）的独特结构，就在表皮板下方。它在哺乳动物I型前庭毛细胞中尤为突出。该提议的中心假设是，横纹细胞器在毛细胞的顶端和基础部分之间提供结构和功能连接。该提案的目标是描述该特定细胞器的三维结构 - AIM 1-，并确定其组成部分（或至少是某些组件） - AIM 2。