A STUDY OF THE STRIATED ORGANELLE IN VESTIBULAR ENDORGANS

前庭恩多根条纹细胞器的研究

• 批准号: 8361921
• 负责人: ANNA LYSAKOWSKI
• 金额: $ 2.47万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-04-01 至 2012-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8361921
• 关键词: Actins; Apical; Architecture; Auditory; Calcium; Cell physiology; Cells; Collaborations; Contractile Proteins; Cytoskeleton; Elements; Feedback; Funding; Goals; Grant; Hair Cells; Image Analysis; Joints; Knowledge; Membrane Potentials; Mitochondria; Modeling; Molecular; Molecular Motors; Motor; National Center for Research Resources; Organelles; Principal Investigator; Proteins; Research; Research Infrastructure; Resources; Sensory; Source; Stereocilium; Structure; Subcellular structure; United States National Institutes of Health; Variant; Vestibular Hair Cells; Vestibular Labyrinth; cost; electron tomography; interest; molecular dynamics; response; sensory mechanism; three dimensional structure

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. In the mammalian vestibular labyrinth, hair cells are capable of generating axial deformations in response to variations in transmembrane potential. Our understanding of electromechanical transduction in the hair cells depends on precise knowledge of intracellular structure, and efforts to model hair cell physiology are likewise limited to the fidelity of known intracellular geometry. This collaboration focuses on the ultrastructure of vestibular hair cells. More precisely, we are looking at the subcuticular region (below the cuticular plate), using electron tomography to visualize and analyze rootlet architecture at the junction of the stereocilia bundle and apical region of the cell. Our main interest is the striated organelle (SO), a unique structure located at the apical end of hair cells (auditory and vestibular sensory cells), just below the cuticular plate. It is particularly prominent in mammalian type I vestibular hair cells. The central hypothesis we are investigating with aid from the NCMIR is that striated organelles provide structural and functional connections between the apical and basal parts of the hair cell. The goals of this joint effort are to describe the three-dimensional structure of this particular organelle and to identify its components (or at least, some of the components). Our study should appeal to: 1) sensory physiologists interested in mechanisms of sensory transduction and mechanotransduction, 2) neuroscientists interested in feedback from afferents to the transduction apparatus, 3) protein chemists interested in potential new motor proteins, contractile elements, calcium dynamics, and the molecular architecture of these mechanisms, 4) molecular and cell biologists interested in actin, molecular motors, the cytoskeleton and mitochondria, and 5) microscopists and morphologists interested in a new and elegant structural apparatus.

该副本是利用资源的众多研究子项目之一 由NIH/NCRR资助的中心赠款提供。对该子弹的主要支持 而且，副投影的主要研究员可能是其他来源提供的 包括其他NIH来源。 列出的总费用可能 代表subproject使用的中心基础架构的估计量， NCRR赠款不直接向子弹或副本人员提供的直接资金。 在哺乳动物的前庭迷宫中，毛细胞能够响应跨膜电位的变化而产生轴向变形。我们对毛细胞中机电转导的理解取决于对细胞内结构的精确知识，并且对毛细胞生理的努力也限于已知细胞内几何形状的忠诚度。这种合作侧重于前庭毛细胞的超微结构。更确切地说，我们正在使用电子断层扫描来查看亚周围区域（在表皮板下方），以可视化和分析细胞立体束和顶端区域的rootlet结构。我们的主要兴趣是横纹细胞器（SO），这是位于毛细胞顶端（听觉和前庭感觉细胞）的独特结构，就在表皮板下方。它在哺乳动物I型前庭毛细胞中尤为突出。我们在NCMIR的帮助下研究的中心假设是，条纹细胞器在毛细胞的顶端和基础部分之间提供结构和功能连接。这项共同努力的目标是描述该特定细胞器的三维结构，并确定其成分（或至少是某些组件）。我们的研究应吸引：1）感官生理学家对感官转导和机械转移机制感兴趣，2）对从传入到转导设备的反馈感兴趣的神经科学家感兴趣的神经科学家，3）蛋白质化学家对潜在的运动蛋白，收缩元素，钙化动力学以及这些机械型的分子型，4）摩尔群的蛋白质化学家感兴趣，4）细胞骨架和线粒体，以及5）对新的优雅结构设备感兴趣的显微镜和形态学家。