Chromosome-Wide Mapping of Recombinational Activity

重组活动的染色体范围图谱

• 批准号: 7299598
• 负责人: Petko M Petkov
• 金额: $ 31.93万
• 依托单位: JACKSON LABORATORY
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-04-01 至 2011-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7299598
• 关键词: biochemical evolution; bioinformatics; chromosomes; computational biology; cytogenetics; functional /structural genomics; gender difference; genetic mapping; genetic recombination; meiosis; species difference

Our goal is to generate the first high-resolution maps of mammalian recombination activity for an entire chromosome as a representative of the genome as a whole, including the variation in hotspot location and activity within and between species, and how this differs in F1 animals from reciprocal crosses and between male and female meiosis. For this we will map recombinants arising in 3000 meioses from each of six genetic crosses at under 50-Kb resolution, enabling us to distinguish individual hotspots and detect activities as low as 0.03 cM/hotspot. These data will go far in helping us understand mammalian recombination as a biological process as well as relationships between parameters of recombination and other aspects of chromosome dynamics. Aim 5a. we will map the location of every crossover on Chromosome 8 arising in six genetic crosses, 3000 offspring each for a total of 18,000 meioses, at under 50 kb resolution. Four of the crosses will be female F1 hybrids created in a daisy-chain design (C57BL/6J x PWD/PhJ x WSB/EiJ x CAST/EiJ x C57BL/6J) and then backcrossed to C57BL/6J. F1 male hybrids from crosses of C57BL/6J x PWD/PhJ and WSB/EiJ x CAST/EiJ will also be backcrossed to C57BL/6J. Whenever possible, equal numbers of meioses will be derived from F1 animals created by reciprocal crosses of the original parents (AxB and BXA). This design is optimal for gathering data on genetic variability, sex specificity, and possible imprinting effects in these crosses. Aim 5b. using the data of aim 5a, we will analyze the strain, sex and cross direction specificity of the location, distribution and activity of the recombination hotspots along the length of Chromosome 8. For understanding evolutionary processes, we will also provide a consensus map of Chromosome 8 hotspots across subspecies. Aim 5c. we will make the 18,000 backcross DMA samples available to the scientific community via a repository, and the 3000 SNP assays that will be newly developed on Chromosome 8 will be posted on our web site and made available to others for QTL and gene mapping studies.

我们的目标是生成整个哺乳动物重组活动的第一张高分辨率地图 染色体作为整个基因组的代表，包括热点位置的变化和 物种内部和之间的活动，以及F1动物与互惠交叉以及之间以及之间的不同 男性和女性减数分裂。为此，我们将绘制从六个遗传中的每一个中的每一个中产生的重组者 以低于50-kb的分辨率进行交叉，使我们能够区分单个热点，并检测到低于 0.03厘米/热点。这些数据将在帮助我们理解哺乳动物重组的过程中远远不足 重组参数与染色体的其他方面之间的过程以及关系 动力学。 目标5A。我们将绘制在六个遗传十字架中出现的每个分频器的位置，3000 后代以低于50 kb的分辨率，总共以18,000倍元。四个十字架将是女性F1 在雏菊链设计中创建的杂种（C57BL/6J X PWD/PHJ X WSB/EIJ X Cast/eij X C57BL/6J），然后 回击向C57BL/6J。 C57BL/6J X X PWD/PHJ和WSB/EIJ X Cast/Eij的F1男性杂种 也将回到C57BL/6J。只要可能 由原始父母（AXB和BXA）的相互交叉创建的动物。此设计是最佳的 收集有关遗传变异性，性别特异性和可能在这些十字架可能产生的烙印效应的数据。 目标5B。使用AIM 5A的数据，我们将分析位置的应变，性别和交叉方向特异性， 重组热点沿染色体8的长度的分布和活动。 进化过程，我们还将提供跨亚种的染色体8热点的共识图。 目标5C。我们将通过一个 存储库以及将在8号染色体上新开发的3000个SNP分析将发布在我们的 网站并提供给其他人进行QTL和基因映射研究。